We narrowed to 18,937 results for: Tec

1. pcDNA3-RAB-AKARev(T/A)

   Plasmid

   #118479

   Purpose

   Negative-control mutant for RAB-AKARev biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-AKARev(T/A)
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. RS18960_pMQ30_KO_Kan

   Plasmid

   #185393

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18960 upstream flanking homology region
   RR42_RS18960 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWB419

   Plasmid

   #74813

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myc-Foxo1-WT 6KQ

   Plasmid

   #17559

   Depositor

   Domenico Accili

   Article

   Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

   Insert

   Foxo1 6KQ (Foxo1 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pGWB522

   Plasmid

   #74864

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2337_Tier3(SB)-TagBFP2_Puro

   Plasmid

   #169635

   Purpose

   Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven mTagBFP2 and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

   Plasmid

   #108375

   Purpose

   FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC167

   Plasmid

   #118909

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMIII-Neo-tdTomato

   Plasmid

   #153424

   Purpose

   Retroviral (MSCV) expression of tdTomato fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   tdTomato
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

    Plasmid

    #192350

    Purpose

    L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    AATG_dttLbCas12a-i_TTCG
    

    Use

    CRISPR

    Mutation

    D156R; E925Q

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-blueCKAR(T/A)

    Plasmid

    #118473

    Purpose

    Negative-control mutant for blueCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1A-LivePAR(R163A)-Hygro

    Plasmid

    #187609

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

    Depositor

    Robert Sobol

    Article

    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

    Insert

    LivePAR(R163A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R163A mutation in the WWE Domain

    Promoter

    EF1A

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB2899(X-2 MarkerFree)

    Plasmid

    #73271

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. mPlum-Gamma-Tubulin-17

    Plasmid

    #55971

    Purpose

    Localization: Centrosomes, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Gamma-Tubulin (TUBG1 Human)
    

    Tags

    mPlum

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCellFree_G03 ANXA1

    Plasmid

    #67071

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ANXA1 (ANXA1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. p3E-GBP

    Plasmid

    #67672

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    anti-GFP nanobody
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CDK4mut(K35M)-3XFlag-pBabe

    Plasmid

    #73559

    Purpose

    vector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tags

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    CDK4mut(K35M)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1P shABCB7_2

    Plasmid

    #102969

    Purpose

    Suppression of ABCB7

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shABCB7_2 (ABCB7 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1P shABCB7_1

    Plasmid

    #102968

    Purpose

    Suppression of ABCB7

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shABCB7_1 (ABCB7 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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