We narrowed to 40,052 results for: lat

1. FKBP-beta1(617-949)-GFP

   Plasmid

   #100732

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP1B1 (AP1B1 Human)
   

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-Stop-EGFP (MSE)

   Plasmid

   #154469

   Purpose

   MSCV-Stop-EGFP-pgk-Neo (retroviral construct containing the RFP-based translational stop cassette)

   Depositor

   Cornelius Miething

   Article

   Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TfR-moxGFP-DHFR

   Plasmid

   #173012

   Purpose

   For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   TfR-moxGFP-DHFR
   

   Tags

   DHFR and moxGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. phage - ubc - nls - 2xmcp - egfp - BirA*

   Plasmid

   #131132

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Article

   Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

   Insert

   NLS-HA-2xMCP-EGFP-BirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

   Plasmid

   #140535

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS TOL2
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 MP1 shRNA

   Plasmid

   #26632

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   MP1 shRNA (LAMTOR3 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pUAST-attB-3xHA-V5-RpL10Ab

   Plasmid

   #125223

   Purpose

   3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

   Depositor

   Herman Dierick

   Article

   Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

   Insert

   RpL10Ab (RpL10Ab Fly)
   

   Tags

   3x HA, V5

   Expression

   Insect

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUMO His6 SUMO PYL1 (36-211)

   Plasmid

   #177843

   Purpose

   Bacterial Expression of PYL1

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 1 (PYL1 Mustard Weed)
   

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-FAM20C:V5

   Plasmid

   #63584

   Purpose

   FAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.

   Depositor

   Kenneth Irvine

   Article

   Ishikawa et al PLoS One. 2012;7(8):e42988. doi: 10.1371

   Insert

   FAM20C (Fam20c Mouse)
   

   Tags

   6xHis and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pSLIK-Hygro-Myc-hAgo2

    Plasmid

    #60234

    Purpose

    Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

    Insert

    Argonaute2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pJZC620

    Plasmid

    #62282

    Purpose

    Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    MCP-VP64
    PCP-VP64
    dCas9
    

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Nuclease activity has been inactivated by mutatio…

    Promoter

    pAdh and pTdh3

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. hGATA-1 K137R

    Plasmid

    #118353

    Purpose

    This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K137R

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PDZb-beta2(616-951)-mCherry

    Plasmid

    #100736

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Tags

    PDZb and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. hHSF1 K298R

    Plasmid

    #118362

    Purpose

    This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K298R

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BMR1_01g02031-bio-His

    Plasmid

    #108116

    Purpose

    [Edit] Expresses enzymatically monobiotinylated full-length BMR1_01g02031 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag

    Depositor

    Gavin Wright

    Article

    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

    Insert

    BMR1_01g02031
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHBD00476

    Plasmid

    #39585

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5838 (Dref Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-ΔN-RNMT

    Plasmid

    #112708

    Purpose

    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Tags

    FLAG and HIV Rev nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    deleted amino acids 2-120, mutated active-site D2…

    Promoter

    CMV

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

    Plasmid

    #79259

    Purpose

    Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    DmeIF4G (eIF4G1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    V5-His6 and lambdaN-HA

    Promoter

    Ac5 promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-dCas9-MQ1(C141S)-EGFP

    Plasmid

    #89635

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNAIIIB rac1 QL

    Plasmid

    #61633

    Purpose

    mammalian expression of rac1 constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    Q61L, constitutively active

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only