We narrowed to 10,134 results for: ada

1. pCMV-dCas12a-ABE8e-V106W

   Plasmid

   #193646

   Purpose

   Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA*8e-V106W, EGFP
   

   Expression

   Mammalian

   Mutation

   TadA*8e-V106W

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPR v2-sgACSL4 clone2

   Plasmid

   #162129

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCRISPR v2-sgACSL4 clone1

   Plasmid

   #162128

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-DCX-TurboID-V5-puro

   Plasmid

   #190742

   Purpose

   A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   DCX-TurboID-V5 (DCX Human, DCX is human doublecortin; TurboID is engineered BirA from E.coli)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1a-hMLH1dn (og codon)

   Plasmid

   #174823

   Purpose

   Mammalian expression of human MLH1dn (original codon usage)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (original codon usage) (MLH1 Human)
   

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-GRAB_UCN1.0

   Plasmid

   #208669

   Purpose

   Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Puro-moxGFP-TUBA1B

   Plasmid

   #207767

   Purpose

   Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pC1-oROS-HT-CaaX

   Plasmid

   #216417

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT-CaaX
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-neo-RL-ZEB2 200bmut

   Plasmid

   #35538

   Depositor

   Greg Goodall

   Article

   Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

   Insert

   3'UTR of ZEB2 gene (ZEB2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   6 Mutations in the miR-200b seed regions of the Z…

   Available Since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163562

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. WDV-Echistatin

    Plasmid

    #226985

    Purpose

    Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

    Depositor

    Wendy Gordon

    Article

    Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

    Inserts

    WDV (repA )
    Echistatin
    

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

    Plasmid

    #163554

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-Anc689-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-shLuc.mKO2

    Plasmid

    #85224

    Purpose

    shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ef1a-DIO-BiPOLES-mCerulean

    Plasmid

    #154949

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1-alpha

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-CyOFP1-TEV

    Plasmid

    #105782

    Purpose

    Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    CyOFP1-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

    Plasmid

    #172104

    Purpose

    Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

    Plasmid

    #176250

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKK-mClover3-TEV

    Plasmid

    #105778

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mClover3-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDisplay-oROS-HT

    Plasmid

    #216416

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    pDisplay-oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-PITCh-PEX3

    Plasmid

    #227303

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PEX3 (PEX3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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