We narrowed to 10,617 results for: t7

1. pCellFree_G06_ZIKV NS4B

   Plasmid

   #145357

   Purpose

   Cell-free expression vector for Zika virus NS4B protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS4B
   

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOmpT

   Plasmid

   #112123

   Purpose

   Bacterial expression outer membrane protein T (OmpT), wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   OmpT (ompT E. coli)
   

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TAL2237

   Plasmid

   #36688

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   CHD4-TALEN_Right (Chd4 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. NmMetQ N238A

   Plasmid

   #129648

   Purpose

   Expresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequence

   Depositor

   Douglas Rees

   Article

   Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p

   Insert

   metQ Gene
   

   Tags

   10x Histidine Tag, Enterokinase, and pelB signal …

   Expression

   Bacterial

   Mutation

   Changed Asparagine 238 to Alanine

   Promoter

   T7 Promoter

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 GTF2B

   Plasmid

   #67067

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2B (GTF2B Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET-DEST42-SARA-V5-His6

   Plasmid

   #67431

   Purpose

   Expression of hSara with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SARA (SAR1B Human)
   

   Tags

   His and V5

   Expression

   Bacterial

   Promoter

   T7/lacO

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. TAL2255

   Plasmid

   #36698

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FANCC-TALEN_Right (Fancc Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCellFree_G03 IFNG

   Plasmid

   #67056

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IFNG (IFNG Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. A104T DJ-1 pET15b

   Plasmid

   #60680

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

   Insert

   A104T DJ-1 pET15b (PARK7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A104T

   Promoter

   T7

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. TAL2278

    Plasmid

    #36713

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MYC-TALEN_Left (Myc Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TAL2279

    Plasmid

    #36714

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MYC-TALEN_Right (Myc Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pOmpW

    Plasmid

    #112121

    Purpose

    Bacterial expression of outer membrane protein W (OmpW), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpW (ompW E. coli)
    

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2-USP44

    Plasmid

    #43893

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

    Insert

    USP44 (USP44 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 or T7

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

14. TAL2315

    Plasmid

    #36741

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FANCF-TALEN_Right (Fancf Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    July 11, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TAL3344

    Plasmid

    #42807

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-prothymosin alpha a-left (ptmaa Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

16. TAL3178

    Plasmid

    #41352

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-sox2-Left (sox2 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-SpyTag002 DA-MBP

    Plasmid

    #102832

    Purpose

    Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyTag002 DA-MBP
    

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Reactive aspartic acid has been mutated to an ala…

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TAL2262

    Plasmid

    #36701

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CTNNB1-TALEN_Left (Ctnnb1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. TAL2263

    Plasmid

    #36702

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CTNNB1-TALEN_Right (Ctnnb1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pET29b_Qcat7

    Plasmid

    #45135

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT7
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge