162,717 results

1. Pflp-18::NGL-1::spGFP11

   Plasmid

   #65828

   Purpose

   For trans-synaptic labeling

   Depositor

   Kang Shen, Cori Bargmann

   Article

   Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

   Insert

   NLG-1 (nlg-1 Nematode)
   

   Use

   Tags

   spGFP11

   Expression

   Worm

   Mutation

   N142S

   Promoter

   Pflp-18

   Available since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Epac-S-H187

   Plasmid

   #170348

   Purpose

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7C-BAG2

   Plasmid

   #177654

   Purpose

   Recombinant protein synthesis of human BAG2

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   BAG2 (BAG2 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Punc-4::NGL-1::spGFP1-10

   Plasmid

   #65827

   Purpose

   For trans-synaptic labeling

   Depositor

   Kang Shen, Cori Bargmann

   Article

   Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

   Insert

   NLG-1 (nlg-1 Nematode)
   

   Use

   Tags

   spGFP1-10

   Expression

   Worm

   Mutation

   Promoter

   Punc-4

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. KA1153_pPBCAG-MerCreMer-IN

   Plasmid

   #124183

   Purpose

   piggyBac-based expression of the tamoxifen-inducible Cre

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   MerCreMer-IRES-Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

   Plasmid

   #166055

   Purpose

   Expresses the full length TurboID on the surface of astrocytes

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LbABE8e(TadA-8e V106W)

   Plasmid

   #138508

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e V106W)-dLbCas12a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TadA-8e V106W

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

   Plasmid

   #185447

   Purpose

   Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells

   Depositor

   Ahmet Yildiz

   Article

   Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

   Inserts

   TRF1 (TERF1 Human)
   POT1 (POT1 Human)
   TPP1 (TPP1 Human)
   TIN2 (TINF2 Human)
   

   Use

   Tags

   MBP, YBBR, and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBacDual with PA+Histag

    Plasmid

    #84870

    Purpose

    Baculovirus Expression of Bacillus anthracis protective antigen

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Insert

    PA
    

    Use

    Baculovirus

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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