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eDrTrkB

Plasmid

#183997

Purpose

Optically controlled eDrTrkB expression in mammalian cells

Depositor

Vladislav Verkhusha, Dmitry Gerashchenko

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkB (NTRK2 Human)

Use

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_346

Plasmid

#180549

Purpose

Entry vector containing gfasPurple

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

gfasPurple

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ265N2

Plasmid

#174001

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-SpRY-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O2

Plasmid

#174002

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265L2

Plasmid

#174000

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-NLSlacZ-stop-L2

Plasmid

#186358

Purpose

Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ Synthetic, E. coli)

Use

Expression of nuclear lacz reporter

Tags

Nuclear localization signal

Expression

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target2 (Mpphot)

Plasmid

#186727

Purpose

Gateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target1 (Mpphot)

Plasmid

#186726

Purpose

Gateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR/Zeo-sfGFP-SKL

Plasmid

#186720

Purpose

Gateway entry vector encoding sfGFP with the two peroxisome-targeting signals (out of frame).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sfGFP-SKL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_316

Plasmid

#180547

Purpose

Entry vector containing sfGFP

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_344

Plasmid

#180548

Purpose

Entry vector containing cjBlue

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

cjBlue

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_345

Plasmid

#180550

Purpose

Entry vector containing eforRed

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

eforRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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MNEAP1ec

Plasmid

#131020

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

MNEAP1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

Promoter

Available since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MKAKU41ec

Plasmid

#131014

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

MKAKU41

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Expression

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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NCH1ec

Plasmid

#131016

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

NCH1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

Promoter

Available since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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NCH2ec

Plasmid

#131018

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

NCH2

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

Promoter

Available since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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KTK_048

Plasmid

#180564

Purpose

Entry vector containing L3S1P00

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P00_E1.4b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_033

Plasmid

#180545

Purpose

Entry vector containing LuxR

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

LuxR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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CPL2g/pDONR221

Plasmid

#172093

Purpose

Genomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

CPL2

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pColE1-AlphaOmega

Plasmid

#165867

Purpose

Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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