We narrowed to 8,600 results for: Set

1. pSF522

   Plasmid

   #222588

   Purpose

   BB3 ZWF1 tet-on overexpression cassette with homologous flanks for integration at pyrG locus

   Depositor

   Matthias Steiger

   Article

   Matthias et al PLOS One

   Insert

   ZWF1
   

   Use

   A. niger

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Promoter

   tet-on

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF8

   Plasmid

   #222592

   Purpose

   BB2 gsdA tet-on overexpression cassette

   Depositor

   Matthias Steiger

   Article

   Matthias et al PLOS One

   Insert

   gsdA
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert,…

   Promoter

   tet-on

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEED-Moontag:HA

   Plasmid

   #219648

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   MoonTag:HA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEED-EYFP:HA

   Plasmid

   #219656

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   EYFP:HA SEED Cassette
   

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKDC.118

   Plasmid

   #227188

   Purpose

   T7 IPTG-driven Cas13a

   Depositor

   Seth Shipman

   Article

   Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

   Insert

   LbuCas13a
   

   Expression

   Bacterial

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJBM2

   Plasmid

   #225289

   Purpose

   E. coli-Campylobacter shuttle vector; KanR

   Depositor

   Robert Moore

   Article

   McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

   Insert

   Replication elements from pCC311 for pJBM2
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLP5

   Plasmid

   #209960

   Purpose

   Entry vector to store Level 0* resistance cassette parts

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLPC-C-SF-TAP-DEST-TLK1- M566T

   Plasmid

   #218831

   Purpose

   Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.

   Depositor

   Travis Stracker

   Insert

   TLK1 M566T (TLK1 Human)
   

   Tags

   FLAG-STREP

   Expression

   Mammalian

   Mutation

   M566T

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCL194

   Plasmid

   #222225

   Purpose

   Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

   Depositor

   Seth Shipman

   Article

   Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   site 1/Cas9/Eco1 RT
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCL195

    Plasmid

    #222226

    Purpose

    Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    site 2/Cas9/Eco1 RT
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCL368

    Plasmid

    #222227

    Purpose

    Expresses site 3/Cas9/Eco1 RT in HIS3 locus.

    Depositor

    Seth Shipman

    Article

    Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    site 3/Cas9/Eco1 RT
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0.425

    Plasmid

    #203944

    Purpose

    Up Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Insert

    Cas12a UP
    

    Use

    Synthetic Biology

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0.426

    Plasmid

    #203945

    Purpose

    Down Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Insert

    Cas12a DOWN
    

    Use

    Synthetic Biology

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA TR KO 2

    Plasmid

    #207610

    Purpose

    sgRNA 2 to knockout TR by replacement with a PuroR cassette

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    TCAGGCCGCAGGAAGAGGAA
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgRNA TR KO 1

    Plasmid

    #207609

    Purpose

    sgRNA 1 to knockout TR by replacement with a PuroR cassette

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    ACCCTAACTGAGAAGGGCGT
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUPD2-HR1bR181A R185A-GFP-L (B3-B4)

    Plasmid

    #211857

    Purpose

    Mutant E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Mutation

    R181A R185A

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid

    #215544

    Purpose

    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

    Depositor

    Kai Wang

    Insert

    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    

    Use

    CRISPR

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDT2

    Plasmid

    #199119

    Purpose

    circular transcription template

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
    

    Expression

    Bacterial

    Mutation

    mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. NK621

    Plasmid

    #196405

    Purpose

    Strong expression of firefly luciferase codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. BASIC_SEVA_69.11

    Plasmid

    #194214

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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