Skip to main content
Addgene
Showing: 6161 - 6180 of 6414 results

  2. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222498
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222498-plasmid-map-sequence-id-441135

    Enlarge

  3. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222499
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222499-plasmid-map-sequence-id-441136

    Enlarge

  4. jk96

    Plasmid
    #120177
    Purpose
    CAG:mcherry:P2A:HA_NLGN
    Depositor
    Anthony Zador
    Insert
    CAG:mCherry:P2A:HA_NLGN
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    120177-plasmid-map-sequence-id-227832

    Enlarge

  12. Aiolos ZF2 (AA146-168) in Cilantro 2

    Plasmid
    #74456
    Purpose
    Monitor post-translational degradation of Aiolos ZF2 via EGFP:mCherry ratio (Lentiviral, PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    74456-plasmid-map-sequence-id-140672

    Enlarge

  15. Aiolos AA130-189 in Pea

    Plasmid
    #74459
    Purpose
    Monitor post-translational degradation of Aiolos AA130-189 via EGFP:mCherry ratio (Lentiviral SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74459-plasmid-map-sequence-id-140419

    Enlarge

  17. mCh-cpFRB1

    Plasmid
    #155389
    Purpose
    Express cpFRB1 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2
    Depositor
    Yubin Zhou
    Article
    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136
    Insert
    cpFRB1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    155389-plasmid-map-sequence-id-315202

    Enlarge

  18. mCh-cpFRB2

    Plasmid
    #159645
    Purpose
    Express cpFRB2 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2
    Depositor
    Yubin Zhou
    Article
    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136
    Insert
    cpFRB2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    159645-plasmid-map-sequence-id-315201

    Enlarge

  19. pCHW100003

    Plasmid
    #222442
    Purpose
    Level 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    mCherry2-HyP5SM
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222442-plasmid-map-sequence-id-454096

    Enlarge

  20. pAP-U2-1

    Plasmid
    #236472
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.
    Insert
    spH2Ap-3xmCherry spH2Bp-3xGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Promoter
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236472-plasmid-map-sequence-id-468916

    Enlarge

Showing: 6161 - 6180 of 6414 results