We narrowed to 14,477 results for: nts

1. pcDNA3.1 3xFLAG TPP1 88-249

   Plasmid

   #53549

   Purpose

   mammalian expression of TPP1 OB-fold domain

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   aa 88-249

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pMOD_A1710

   Plasmid

   #91039

   Purpose

   Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-TaCas9_H840A nickase
   

   Use

   CRISPR

   Mutation

   H840A

   Promoter

   ZmUbi

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

   Plasmid

   #91873

   Purpose

   Photo-activatable/light sensitive Vav2

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   VAV2 DH/PH/ZF (Vav2 Mouse)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE663

   Plasmid

   #153261

   Purpose

   M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGE665

   Plasmid

   #153263

   Purpose

   M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGE654

   Plasmid

   #153252

   Purpose

   M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

   Plasmid

   #192681

   Purpose

   Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-SLC30A10-E25A

   Plasmid

   #82345

   Purpose

   Expresses FLAG-tagged SLC30A10-E25A in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

   Insert

   SLC30A10 (SLC30A10 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   E25A

   Promoter

   CMV

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. E1_pENTR_L1-L4_NI_G1397-DddtoxA-C

   Plasmid

   #171726

   Purpose

   ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
   

   Use

   Multisite gateway entry vector

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. E3_pENTR_L3-L2_NG_G1397-DddtoxA-C

    Plasmid

    #171734

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. E3_pENTR_L3-L2_NG_G1397-DddtoxA-N

    Plasmid

    #171735

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. E1_pENTR_L1-L4_NI_G1397-DddtoxA-N

    Plasmid

    #171727

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. E1_pENTR_L1-L4_NN_G1397-DddtoxA-N

    Plasmid

    #171731

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. E1_pENTR_L1-L4_NN_G1397-DddtoxA-C

    Plasmid

    #171730

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTaCas9-D10A_5

    Plasmid

    #91170

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A (nickase)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 2Bc-T MBP_BoMoC(ed)_I770A_6xH

    Plasmid

    #185711

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed I770 to A

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cdc42 T17N sp64T

    Plasmid

    #16672

    Depositor

    De-Li Shi

    Article

    Djiane et al Development. 2000 Jul . 127(14):3091-100

    Insert

    Cdc42 T17N (CDC42 Human)
    

    Use

    Xenopus expression

    Mutation

    T17N. Dominant negative.

    Available Since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

18. phNFL-P440L

    Plasmid

    #132596

    Purpose

    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFL (NEFL Human)
    

    Expression

    Mammalian

    Mutation

    1319C>T; Pro440Leu

    Promoter

    CMV

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1 Insulator ObLiGaRe/EPB71

    Plasmid

    #90018

    Purpose

    Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

    Depositor

    Eric-Paul Bennett

    Article

    Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

    Insert

    MCS flanked by insulator and inverted ZFN binding sites
    

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX4T2-Snf1-cat

    Plasmid

    #52683

    Purpose

    expression of the Snf1 kinase domain in bacteria

    Depositor

    Benjamin Turk

    Article

    Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

    Insert

    SNF1 (SNF1 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes kinase domain only, aa 41-315

    Promoter

    tac

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only