Addgene

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pCMV-Myc-rSyntenin1

Plasmid

#89435

Purpose

mammalian expression of rat Syntenin1

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV ORANGE Gria1-HaloTag KI

Plasmid

#139655

Purpose

Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and HaloTag donor

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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CACNA2D1-TwinStrepII

Plasmid

#83192

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Insert

CACNA2D1 (Cacna2d1 Rat)

Use

Tags

Twin Strep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pSV Sport SREBP-1c dom neg

Plasmid

#8885

Depositor

Bruce Spiegelman

Article

Kim et al Genes Dev. 1996 May 1. 10(9):1096-107.

Insert

SREBP-1c (Srebf1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Y305A amino acid substitution in the active versi…

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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mGeos-M-Homer1-C-18

Plasmid

#57528

Purpose

Localization: Membrane Adaptor, Excitation: 503 / 569, Emission: 514 / 581

Depositor

Michael Davidson

Insert

Homer1 (Homer1 Rat)

Use

Tags

mGeos-M

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-SYN_mEGFP-CREB

Plasmid

#137006

Purpose

AAV backbone, mEGFP-CREB donor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

AAV

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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mMaple-OMP25

Plasmid

#141151

Purpose

Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Use

Tags

mMaple

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30-rSyntenin1

Plasmid

#89428

Purpose

bacterial expression of rat Syntenin1

Depositor

Fritz Rathjen

Insert

SDCBP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T5

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG.PKCzeta.K/W

Plasmid

#10800

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K281W (Lys 281 to Trp)

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pLenti6-AMPK alpha1 (1-312)

Plasmid

#162131

Purpose

Expression vector for constitutively active AMPK alpha 1

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

Insert

AMPK alpha1 (1-312) (PRKAA1 Rat)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP7c variant 1513-WPRE

Plasmid

#105321

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV1

Insert

jGCaMP7c variant 1513

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pBK-YE1-BE3

Plasmid

#85174

Purpose

Expresses YE1-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YE1-BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mCherry-KIX-mCherry

Plasmid

#137007

Purpose

AAV backbone, mCherry-KIX acceptor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

KIX domain (Crebbp Rat)

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2BmGreenLantern

Plasmid

#177332

Purpose

To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

Depositor

Gregory Petsko, Pantelis Tsoulfas, Murray Blackmore

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

H2B (Hist1h2bq Rat)

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Mutation

This fusion protein was optimized to the Human co…

Promoter

CAG

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 barr1 HA

Plasmid

#14693

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-CIB1-mRFP1-MP

Plasmid

#58367

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Use

Tags

mRFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pBK-YEE-BE3

Plasmid

#85177

Purpose

Expresses YEE-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-mCREB

Plasmid

#68551

Purpose

AAV expression of mCREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

mCREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

S133A

Promoter

CMV

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
RnBarr2-mCherry

Plasmid

#137794

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCAX DAB1

Plasmid

#30139

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Disabled-1 (Dab1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only