2,294 results

1. pET-28a-His6-SUMO-Munc18-1wt

   Plasmid

   #135550

   Purpose

   Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18-1wt
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-No-HaloTag7-LC3

   Plasmid

   #184900

   Purpose

   Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Tags

   HaloTag7

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP

   Plasmid

   #34631

   Depositor

   Wolfhard Almers

   Article

   Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

   Insert

   rat Syntaxin1a (Stx1a Rat)
   

   Tags

   meGFP

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenSyn2-EGFP-­OMP25C

   Plasmid

   #71427

   Purpose

   Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

   Depositor

   Henri Huttunen

   Article

   Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

   Insert

   OMP25 (172-­‐206) (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 172-206 only

   Promoter

   synapsin

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMXs-puro GFP-b5 ER

   Plasmid

   #38274

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Autophagy. 2010 Aug;6(6):764-76.

   Insert

   Cytochrome b5 (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Cyb5a Tranmembrane region from 95 to 134 amino ac…

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

6. AAV-mDlx-jGCaMP8s-WPRE

   Plasmid

   #176755

   Purpose

   jGCaMP8s expression in GABA-ergic interneurons under the control of mDlx enhancer

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   mDlx

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. EBFP2-Lysosomes-20

   Plasmid

   #55246

   Purpose

   Localization: Lysosome Membrane, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Insert

   LAMP1
   

   Tags

   EBFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. N-WASP

   Plasmid

   #186132

   Purpose

   For expression of recombinant N-WASp in bacteria cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   WASL (WASL Rat)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Amino Acid residues 183-193 fused to 273-501

   Promoter

   T7

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre

   Plasmid

   #134308

   Purpose

   Expresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)

   Depositor

   Elly Nedivi

   Article

   Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

   Inserts

   Synaptophysin (Syp Mouse, Rat)
   Cre-ER
   

   Tags

   TdTomato

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. myc-mTOR (1271-2008 aa)

    Plasmid

    #21746

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    mTOR (1271-2008) (Mtor Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    amino acids 1271-2008

    Available Since

    Aug. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pCR-XL-rAkap9

    Plasmid

    #196867

    Purpose

    Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akap9 (Akap9 Rat)
    

    Expression

    Bacterial

    Promoter

    Lac promoter and T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-MPAct-mCit-IRES-Neo

    Plasmid

    #155230

    Purpose

    lentiviral MPAct construct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lentiviral expression of MPAct-mCitrine sensor
    

    Use

    Lentiviral

    Tags

    mCitrine

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORANGE Bsn-GFP KI

    Plasmid

    #139665

    Purpose

    Endogenous tagging of Bassoon: C-terminal (amino acid position: F3938)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PKC beta I DN

    Plasmid

    #16381

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC beta 1 (Prkcb Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative (kinase inactive): K371R

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. VAChT-pHuji

    Plasmid

    #105319

    Purpose

    VAChT tagged with pH-sensitive red fluorescent protein pHuji

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAChT (Slc18a3 Rat)
    

    Tags

    pHuji

    Expression

    Mammalian

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-beta-arrestin2

    Plasmid

    #36919

    Depositor

    Robert Lefkowitz

    Article

    Xiao et al J Biol Chem. 2004 Dec 31;279(53):55744-5

    Insert

    beta-arrestin2 (Arrb2 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1_rSERT_WT

    Plasmid

    #190743

    Purpose

    For expression of wild-type rat SERT in mammalian cells

    Depositor

    Gary Rudnick

    Article

    Singh et al bioRxiv 2022.06.13.495991

    Insert

    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBactin-PACT-mOrange2-hCM1

    Plasmid

    #196868

    Purpose

    Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    PACT-mOrange2-hCM1 (Akap9 Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV5-rat ERK2-L73P/S151D

    Plasmid

    #40819

    Depositor

    Natalie Ahn

    Article

    Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

    Insert

    MAPK1 (Mapk1 Rat)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    L73P, S151D

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

    Plasmid

    #105323

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7c variant 1513
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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