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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,322 results for: pET28a

Showing: 901 - 930 of 1322 results

  1. WDR5 (2H9M)

    Plasmid
    #25228
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5_01 (WDR5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. phSOD1-I113T-N’His

    Plasmid
    #117703
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    I113T
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117703-plasmid-map-sequence-id-227513

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  3. UPLC1

    Plasmid
    #25550
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UPLC1 (ASAP3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. CAPN9 (1ZIV)

    Plasmid
    #25617
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CAPN9 (CAPN9 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains catalytic domain
    Promoter
    Available Since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. phSOD1-E100G-N’His

    Plasmid
    #117706
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    E100G
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117706-plasmid-map-sequence-id-227528

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  6. ARL10B:L9-K182

    Plasmid
    #25523
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ARL10B:L9-K182 (ARL8A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids L9-K182
    Promoter
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pET28-Flag-RBM24-1~93

    Plasmid
    #208633
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 1~93, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208633-plasmid-map-sequence-id-415921

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  8. pET28-Flag-RBM24-95~236

    Plasmid
    #208634
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 95~236, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208634-plasmid-map-sequence-id-418349

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  9. pHS29

    Plasmid
    #171205
    Purpose
    bacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motif
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    meg-3 (meg-3 Nematode)
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    F705A,Y708A,Y713A, N725A
    Promoter
    Available Since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171205-plasmid-map-sequence-id-335784

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  10. phSOD1-L126X-N’His

    Plasmid
    #117707
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    L126X
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117707-plasmid-map-sequence-id-227541

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  11. phSOD1-D101G-N’His

    Plasmid
    #117704
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D101G
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117704-plasmid-map-sequence-id-227520

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  12. pJK652

    Plasmid
    #72470
    Purpose
    Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)
    Depositor
    T. J. Kappock
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated histidine-233 to glycine
    Promoter
    T7
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72470-plasmid-map-sequence-id-134018

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  13. pJK522

    Plasmid
    #72510
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72510-plasmid-map-sequence-id-132535

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  14. WDR5 (2H9O)

    Plasmid
    #25230
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5_01 (WDR5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. HOXD1 (murine, partial) HIS-tag pET

    Plasmid
    #8560
    Depositor
    Corey Largman
    Insert
    HOXD1 (Hoxd1 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    Partial protein containing 10 AA N-terminal to HD…
    Promoter
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  20. WT FL USP7

    Plasmid
    #227214
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227214-plasmid-map-sequence-id-452331

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  25. EHMT2

    Plasmid
    #25503
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EHMT2 (EHMT2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. CAPN1

    Plasmid
    #25607
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CAPN1 (CAPN1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 29-360
    Promoter
    Available Since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. MYST3

    Plasmid
    #25181
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST3_13 (KAT6A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains catalytic region ( residues 497-780).
    Promoter
    Available Since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. FKBP8

    Plasmid
    #25609
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FKBP8 (FKBP8 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    FKBP-type PPIase Domain, residues 90-205
    Promoter
    Available Since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25609-plasmid-map-sequence-id-454823

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  29. GCN5L2

    Plasmid
    #25482
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GCN5L2 (KAT2A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 901 - 930 of 1322 results