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mCherry-CRY2clust

Plasmid

#105624

Purpose

Expresses improved CRY2PHR homo-oligomerization

Depositor

Won Do Heo

Article

Park et al Nat Commun. 2017 Jun 23;8(1):30. doi: 10

Insert

mCherry-CRY2PHR-9aa (CRY2 Human, Mustard Weed)

Use

Tags

9 amino acids

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pOmniBac zz TEV YBBR TRF2

Plasmid

#185443

Purpose

Expresses human TRF2 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Insert

TRF2 (TERF2 Human)

Use

Tags

YBBR and ZZ

Expression

Insect

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(1)-CRY2-mCherry

Plasmid

#160440

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(0)-CRY2-mCherry

Plasmid

#160439

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C2_14

Plasmid

#60290

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

EDARADD inactive enhancer (EDARADD Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C2_14

Plasmid

#60253

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

EDARADD inactive enhancer (EDARADD Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pOPO646

Plasmid

#195307

Purpose

pBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

TnsD of McCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Arabinose inducible arapBAD

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII HSPB15

Plasmid

#61039

Purpose

Probe for HSPB51b/15 for in situ hybridization in zebrafish

Depositor

Lara Hutson

Article

Marvin et al Dev Dyn. 2008 Feb;237(2):454-63.

Insert

HSPB15 (hspb15 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-Noggin

Plasmid

#163586

Purpose

Expresses Noggin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Nog (Nog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par6B

Plasmid

#15473

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 6B (Pard6b Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pk-myc-Par6A

Plasmid

#15472

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 6A (Pard6g Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pCRII HSPB12

Plasmid

#61037

Purpose

Probe for HSPB12 for in situ hybridization in zebrafish

Depositor

Lara Hutson

Article

Marvin et al Dev Dyn. 2008 Feb;237(2):454-63.

Insert

HSPB12 (hspb12 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
p3XFLAG-MKL2

Plasmid

#27175

Purpose

For mammalian expression of human MKL2 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Selvaraj et al J Biol Chem. 2003 Oct 24. 278(43):41977-

Insert

MKL2 (MRTFB Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCAFNF-LifeAct-EGFP

Plasmid

#163608

Purpose

Expresses LifeAct fused to EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

LifeAct

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG SARS-CoV2 RdRp.co

Plasmid

#154008

Purpose

Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

Depositor

Patrick Moore

Article

Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

Insert

Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)

Use

Tags

3xFLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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SpyTag-RBD Omicron BQ.1.1

Plasmid

#214725

Purpose

Expresses SARS-CoV-2 Omicron BQ.1.1 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Use

Tags

SpyTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Rac1

Plasmid

#163607

Purpose

Expresses Rac1 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rac1 (Rac1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Rhoa

Plasmid

#163605

Purpose

Expresses RhoA Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rhoa (Rhoa Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Alpha

Plasmid

#177961

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )

Use

Tags

Expression

Mammalian

Mutation

del69, 70, del144, D614G, N501Y, A570D, P681H, T7…

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

Plasmid

#70668

Purpose

Human mutant of E2F1 lacking amino acids 2 to 126

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 2 to 126 (E2F1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

lacks amino acids 2-126

Promoter

cmv

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only