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pET30a-SARS-CoV2-ORF8

Plasmid

#165089

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MBC2-PuroIII

Plasmid

#153420

Purpose

MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230

Plasmid

#225516

Purpose

Lentiviral TMEM230 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(R78L)

Plasmid

#225517

Purpose

Lentiviral TMEM230(R78L) expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

R78L

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(X121PG)

Plasmid

#225518

Purpose

Lentiviral TMEM230(X121PG) expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

X121PG

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(X121W)

Plasmid

#225519

Purpose

Lentiviral TMEM230(X121W) expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

X121W

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(Y29C)

Plasmid

#225520

Purpose

Lentiviral TMEM230(Y29C) expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

Y29C

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230triplemut

Plasmid

#225521

Purpose

Lentiviral TMEM230triplemut expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

Y92C/R141L/X184W

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -3D+3N

Plasmid

#234614

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_3DN (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214N, D242N, D250N

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -4D+4N

Plasmid

#234611

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4DN (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214N, D242N, D250N, D262N

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -4D+4V

Plasmid

#234610

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4DV (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214V, D242V, D250V, D262V

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -3D+3V

Plasmid

#234613

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_3DV (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214V, D242V, D250V

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBS CMV GAG

Plasmid

#234992

Purpose

For production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cells

Depositor

Daniel Leahy, Monica Gonzalez-Magaldi

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

GAG

Use

Tags

Expression

Mammalian

Mutation

Deleted polymerase coding sequence

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

Plasmid

#223174

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA

Expression

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FRB-Fis1 (no mt-mKeima)

Plasmid

#223795

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

FRB-Fis1

Use

Lentiviral

Tags

FRB

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_WIPI2d-TEV-GST

Plasmid

#223799

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens, James Hurley

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI2d (WIPI2 Human)

Use

Tags

TEV-GST

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_GST-TEV-WIPI3

Plasmid

#223800

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens, James Hurley

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI3 (WDR45B Human)

Use

Tags

GST-TEV

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Hyg-ADE2

Plasmid

#232104

Purpose

Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

Insert

ADE2 gRNA and repair template

Use

CRISPR

Tags

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

GAL7

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Kan-ADE2

Plasmid

#232105

Purpose

Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

Insert

ADE2 gRNA and repair template

Use

CRISPR

Tags

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

GAL7

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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FAM177A1-Halo

Plasmid

#224591

Purpose

For expression of FAM177A1-Halo in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Use

Tags

HALO

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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