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pAB042b

Plasmid

#79193

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-MBPoff7

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAB042d

Plasmid

#79194

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-p38_2_3

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAB043a

Plasmid

#79222

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-MBP; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

April 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAB082f

Plasmid

#79238

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d23A

Expression

Bacterial

Promoter

Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET15-b_holD-GS12-holC

Plasmid

#254005

Purpose

T7 expression vector encoding E. coli ψ-GS12-χ fusion for biochemical reconstitution and clamp loader studies.

Depositor

Linda Bloom

Article

Padgett-Pagliai et al Nucleic Acids Res. 2026 May 20;54(10):gk

Insert

holD-GS12-holC (ψ-GS12-χ fusion)

Expression

Bacterial

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_holD-GS12-holC_holB

Plasmid

#254009

Purpose

T7 co-expression vector encoding ψ-GS12-χ fusion (MCS1) and holB (MCS2) for clamp loader reconstitution.

Depositor

Linda Bloom

Article

Padgett-Pagliai et al Nucleic Acids Res. 2026 May 20;54(10):gk

Insert

holD-GS12-holC + holB

Expression

Bacterial

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pTL52_pyCAG-s36GFPHaloTag-IRES-mCherry3NLS-polyA

Plasmid

#223544

Purpose

Minimal PUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, no insulation or mammalian selection gene

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-HaloTag, mCherry-3NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pTL53_pyCAG-s36GFPmNG-IRES-mCherry3NLS-polyA

Plasmid

#223545

Purpose

Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by IRES, strong constitutive promoter CAG, no insulation or mammalian selection gene

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-mNeonGreen, mCherry-3NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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JDW 1061 (pLenti6.2_CMV_GATA1_P2A_H2A_mCherry)

Plasmid

#233543

Purpose

A CMV driven human GATA1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Tags

V5

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1394 (pME-ZIC3-P2A-H2A-mCherry)

Plasmid

#232133

Purpose

A middle entry gateway clone containing V5 tagged human ZIC3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

ZIC3 (ZIC3 Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#233545

Purpose

A CMV driven human MAFB followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Lentiviral

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGU06

Plasmid

#212148

Purpose

Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Insert

alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Agx1: S. cerevisiae; alaA, tyrB: E. coli, Budding Yeast)

Expression

Bacterial

Promoter

PgyrA

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPPC031

Plasmid

#171152

Purpose

pGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassette

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J3-BBa_J23117-mRFP

Expression

Bacterial

Promoter

J3-BBa_J23117

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC034

Plasmid

#171153

Purpose

pGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassette

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J3(106)- BBa_J23111-sfGFP

Expression

Bacterial

Promoter

J3(106)-BBa_J23111

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFE-sfGFP

Plasmid

#162688

Purpose

pFE vector control expressing sfGFP

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

sfGFP

Tags

C terminal 6x His tag

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAG369-lyn11-redFAST

Plasmid

#160356

Purpose

Expresses Lyn11-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG361-lyn11-greenFAST

Plasmid

#160352

Purpose

Expresses Lyn11-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSB4C5_J23101-vdh

Plasmid

#128130

Purpose

Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

vdh

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-HipO

Plasmid

#128133

Purpose

The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

HipO

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAB042a

Plasmid

#79192

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-YSX1

Expression

Bacterial

Promoter

Plac62; Ptet

Availability

Academic Institutions and Nonprofits only

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