We narrowed to 852 results for: VILL

1. LentiV_Neo_POU2F3_CR

   Plasmid

   #122175

   Purpose

   Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

   Insert

   POU2F3 (POU2F3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   3 silent mutations for gRNA resistance (882g-c, 8…

   Promoter

   EFS

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1 hSCN5A_deltaSIV

   Plasmid

   #145771

   Purpose

   expresses human Nav1.5 lacking the SIV motif in mammalian cells

   Depositor

   Hugues Abriel

   Article

   Jespersen et al Biochem Biophys Res Commun. 2006 Oct 6;3

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Stop codon at Serine 2013

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-AUP1-FLAG 5E

   Plasmid

   #185341

   Purpose

   Assessing G2BR function in AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   Flag, 6xHis

   Expression

   Mammalian

   Mutation

   R382E/Q383E/K390E/R398E/R400E

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-AUP1-FLAG DTM

   Plasmid

   #185337

   Purpose

   Assessing domain requirements of AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 24-45, corresponding to the hairpi…

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Chr1q_Cassette-Integration_gRNA

   Plasmid

   #195126

   Purpose

   gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr1q gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-CMV-XRCC1-EGFP-Hygro

   Plasmid

   #176062

   Purpose

   EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   X-ray repair cross complementing 1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-TetOne-Puro-PFDN2

   Plasmid

   #202664

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   prefoldin subunit 2 (PFDN2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCMV-intron myc Rab11 S25N

   Plasmid

   #46786

   Purpose

   expression of S25N Rab11a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB11A S25N (RAB11A Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S25N dominant negative

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pECE-M2-AMPKalpha2 wt

   Plasmid

   #31652

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

10. Rab8-T22N(Flag) in pcDNA3.1neo

    Plasmid

    #46784

    Purpose

    expression of T22N Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A T22N (RAB8A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T22N dominant negative

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. LentiV_Neo_SIK3_CR_T221E

    Plasmid

    #108108

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation),…

    Promoter

    EFS promoter

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-CDC27 wt

    Plasmid

    #31673

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    cell division cycle 27 homolog (CDC27 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

13. OR2M4_Deletion_gRNA

    Plasmid

    #195194

    Purpose

    dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR2M4 dual gRNA (OR2M4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRAF_S259A_pcDNA3.1

    Plasmid

    #86505

    Purpose

    Expresses CRAF_S259A in mammalian cells

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S259A

    Promoter

    CMV

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-6P-1 AUP1 292-410

    Plasmid

    #185331

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 292-410 only

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL-p21UTRm1&2

    Plasmid

    #20880

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 1 (Position ~420) GC…

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

    Plasmid

    #107163

    Purpose

    Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme

    Depositor

    Jose Carlos Cameselle

    Article

    Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.

    Insert

    F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pECE-M2-BAIAP2 S366A

    Plasmid

    #31657

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 S366A (BAIAP2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 366 to Alanine

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

19. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. LRG/Foxa1 e2.4

    Plasmid

    #105511

    Purpose

    Lentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNA

    Depositor

    Christopher Vakoc

    Article

    Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

    Insert

    Foxa1 (sgRNA, e2.4)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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