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pBbdCas9S(RBSmut)_arr2

Plasmid

#149575

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9B(RBSmut)_arr2

Plasmid

#149579

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9H(RBSmut)_arr2

Plasmid

#149580

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKM54

Plasmid

#154157

Purpose

Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB site

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Promoter

8 copies of the Aedes aegypti Rel2 NF-κB site

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pKM44

Plasmid

#154153

Purpose

Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Promoter

Drosophila melanogaster minimal Hsp70 promoter

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJK359

Plasmid

#71570

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctA)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK286

Plasmid

#71567

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (UctB)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

formyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK358

Plasmid

#71569

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctD)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETC-PITPbeta (splice variant 1) K61A

Plasmid

#58271

Purpose

bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-TFEB

Plasmid

#38119

Purpose

Mammalian expression of Transcription factor EB fused to EGFP

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-A

Plasmid

#38132

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-A (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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6xAP-1_RE::Renilla

Plasmid

#178317

Purpose

AP-1 Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

AP-1 Renilla Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-M

Plasmid

#38131

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-M (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-D

Plasmid

#38133

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-D (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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2xP53_RE::FLuc

Plasmid

#178316

Purpose

P53 Firefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

P53 FLuc Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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7xSMAD_RE::GrRenilla

Plasmid

#178319

Purpose

SMAD GreenRenilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

SMAD GreenRenilla Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

Plasmid

#132962

Purpose

Firefly-luciferase single-strand annealing assay cloning backbone

Depositor

Gang Bao

Article

Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

Insert

Firefly Luciferase

Use

CRISPR and Luciferase

Expression

Mammalian

Mutation

Stop sequence between luciferase gene

Promoter

CMV

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNA500

Plasmid

#149576

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

Plasmid

#51473

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6m

Use

AAV

Promoter

hSyn1

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pNLuc

Plasmid

#118058

Purpose

GBPart - NLuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Nano Luciferase

Use

Luciferase and Synthetic Biology; Gbpart

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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