We narrowed to 1,665 results for: plasmid for e coli

1. pET/His-Rab1a(Q70L)

   Plasmid

   #232919

   Purpose

   E. coli Expression

   Depositor

   James Hurley

   Insert

   RAB1A (RAB1A Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Q70L

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPL5071_TEF1*-Cre_URA3

   Plasmid

   #60930

   Purpose

   Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Use

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Mutation

   Promoter

   mutant TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPL5606_TEF1*-Cre_ADE2

   Plasmid

   #60932

   Purpose

   Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Use

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Mutation

   Promoter

   mutant TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPL5608_TEF1*-Cre_TRP1

   Plasmid

   #60931

   Purpose

   Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Use

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Mutation

   Promoter

   Mutant TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. G555

   Plasmid

   #216483

   Purpose

   Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

   Depositor

   Christophe Danelon

   Insert

   plsB, plsC, cdsA, pssA
   

   Use

   Cell-free expression

   Tags

   Expression

   Mutation

   Promoter

   T7 for each gene

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPL5628_TEF1*-Cre_MET15

   Plasmid

   #60933

   Purpose

   Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Use

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Mutation

   Promoter

   mutant TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pECT3

   Plasmid

   #75450

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pECT5

   Plasmid

   #75451

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pECM1

   Plasmid

   #75445

   Purpose

   Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pECT1

    Plasmid

    #75446

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pECT1B

    Plasmid

    #75447

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pECT1D

    Plasmid

    #75448

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pECT1E

    Plasmid

    #75449

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-ITR-EcYtR(NGS-6)-mCherry

    Plasmid

    #217363

    Purpose

    Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    E. coli tyrosine tRNA mutant, "NGS-6"
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV(MinDis).iGluSnFR

    Plasmid

    #41732

    Purpose

    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Insert

    GltI based Glutamate Biosensor
    

    Use

    Glutamate biosensor

    Tags

    Myc, PDGFR transmembrane helix (aa 513-561), and …

    Expression

    Mammalian

    Mutation

    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

    Promoter

    CMV

    Available since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a_SYK

    Plasmid

    #224240

    Purpose

    Expression of human SYK Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    syk (SYK Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sc FLAG-rad24-pRSFDuet

    Plasmid

    #240733

    Purpose

    Over-express FLAG-rad24 in E. coli

    Depositor

    Michael O'Donnell

    Insert

    FLAG-rad24 (RAD24 Budding Yeast)
    

    Use

    Tags

    3X FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET/His-GST-Rab1a(Q70L)

    Plasmid

    #232911

    Purpose

    E. coli Expression

    Depositor

    James Hurley

    Insert

    RAB1A (RAB1A Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Q70L

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pG2K

    Plasmid

    #71742

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge