We narrowed to 8,865 results for: APP

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pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231363

Purpose

Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD 5W D262V

Plasmid

#234617

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_5W D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

F222W, F228W, Y237W, D262V, Y305W, F320W

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-hCPT2-Halo

Plasmid

#232464

Purpose

Expresses Halo tagged CPT2 in neurons

Depositor

Timothy Ryan

Article

Kumar et al Nat Metab. 2025 Jul;7(7):1392-1403. doi:

Insert

CPT2 (CPT2 Human)

Use

Lentiviral

Tags

Halo

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-MYC-As-NF-kB3

Plasmid

#180205

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB3

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-412

Promoter

CMV

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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FGF8-P2A-iCre

Plasmid

#210800

Purpose

Recombinase reporter for FGF8 expression

Depositor

Yuejun Chen

Article

You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

Insert

FGF8 (FGF8 Human)

Use

Cre/Lox

Tags

iCre

Expression

Mammalian

Promoter

endougeneous FGF8 promoter

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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APCDD1-P2A-tdTomato

Plasmid

#210801

Purpose

Fluorescent reporter for APCDD1 expression

Depositor

Yuejun Chen

Article

You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

Insert

APCDD1 (APCDD1 Human)

Tags

tdtomato

Expression

Mammalian

Promoter

endougeneous APCDD1 promoter

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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T40A+Q54A

Plasmid

#217913

Purpose

A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP T40A+Q54A

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Threonine at position 41 and 253 mutated to Alani…

Promoter

T5

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-As-NF-kB2

Plasmid

#180201

Purpose

Expresses FLAG-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-502

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Tags

ALFA tag

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-G_LF(C199S)

Plasmid

#216116

Purpose

Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

oROS-G_LF(C199S)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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OptoProfilin.S138E

Plasmid

#208287

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138E

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin.S138E (Cry2, PRF1 Mouse, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Glu

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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OptoProfilin.S138A

Plasmid

#208288

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138A

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin (Cry2, PRF1 Mouse, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Ala

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

Plasmid

#207054

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

L55W T56A V61W R65A

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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L_NLS-mutNLS V5-Twist

Plasmid

#201381

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

(NLS)SV40A4-V5

Expression

Mammalian

Mutation

K38R K73R

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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