We narrowed to 17,209 results for: EMB

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pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mYFP

Plasmid

#105141

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mYFP

Tags

41nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA1

Plasmid

#46789

Purpose

To express Fun30 in trans in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Promoter

PGAL1

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only
pCS2-AcrIIA4

Plasmid

#174034

Purpose

In vitro transcription of anti-CRISPR AcrIIA4 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA4

Use

In vitro transcription

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA2

Plasmid

#174033

Purpose

In vitro transcription of anti-CRISPR AcrIIA2 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA2

Use

In vitro transcription

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pWZ414-F55

Plasmid

#23079

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R …

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pTAL4

Plasmid

#31035

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALEN-FokI nuclease backbone

Tags

AcV5 and FokI nuclease

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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TAL2453

Plasmid

#36842

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

RUNX1-TALEN_Right (Runx1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pmCherry-C1-DH

Plasmid

#67898

Purpose

Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-DH

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcryBFP_Umyo18bGFP

Plasmid

#172527

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.

Depositor

Joachim Berger

Article

Berger et al Hum Mol Genet. 2017 Mar 15;26(6):1146-11

Insert

myo18b

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-7aa-MAO-pEGFP-C3

Plasmid

#166765

Purpose

To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

Depositor

David Andrews

Insert

MAO tail anchor (MAOA Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-1-830

Plasmid

#40963

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 1-830

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
PUC9B71A

Plasmid

#44602

Depositor

Robert Oshima

Article

Singer et al J Biol Chem. 1986 Jan 15;261(2):538-47.

Insert

endoB (Krt18 Mouse)

Expression

Bacterial

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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C512-E04: attB5r-mCherry-nostop-attB1r

Plasmid

#162941

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-04

Plasmid

#119983

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:ER-GFP1–10

Plasmid

#125664

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-Scs2

Plasmid

#58834

Purpose

Expresses GFP-Tagged Scs2 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Scs2 (SCS2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pML1

Plasmid

#108332

Purpose

MIDAS Level-1 source vector for cloning of modules (synthetic biology parts)

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMMRSF1-16

Plasmid

#20177

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

src homology domain 3 (CRK Human)

Tags

His and NpuDnaEC16

Expression

Bacterial

Available Since

Aug. 17, 2009

Availability

Academic Institutions and Nonprofits only

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XE3 Xwnt-5A Full Length

Plasmid

#16875

Depositor

Randall Moon

Article

Moon et al Development. 1993 Sep. 119(1):97-111.

Insert

Xwnt-5A (wnt5a.L Frog)

Use

Xenopus expression

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only