We narrowed to 29,795 results for: REP

1. pLenti GW-NL-myc

   Plasmid

   #113455

   Purpose

   Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Tags

   NanoLuc-cmyc

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2 Notch1 LNG-6MT

   Plasmid

   #41738

   Depositor

   Raphael Kopan, Jeffrey Nye

   Article

   Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

   Insert

   Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   Contains murine Notch-1 leader peptide (aa 1-23) …

   Promoter

   Available Since

   Jan. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5FRTTO-NHASt-codoptUL50

   Plasmid

   #135101

   Purpose

   Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL50 codon optimized
   

   Use

   Tags

   HA-Strep-Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LSB-hsa-miR-133a-3p

   Plasmid

   #103221

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-133a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-133a-3p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 PA-NL

   Plasmid

   #113445

   Purpose

   ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-NanoLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 PA-mCit-NL

   Plasmid

   #113444

   Purpose

   ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-mCitrine-NanoLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Plasmid

   #101233

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant G582A/V583A/E584A/D585A/N588A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   G582A, V583A, E584A, D585A and N588A

   Promoter

   T7

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LSB-hsa-miR-26a-5p

   Plasmid

   #103379

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-26a-5p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LSB-hsa-miR-125b-5p

   Plasmid

   #103191

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-125b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-125b-5p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LSB-hsa-miR-101-5p

    Plasmid

    #103166

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-101-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-101-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5FRTTO-NHASt-codoptUL23

    Plasmid

    #135104

    Purpose

    Codon-optimized HSV-1 UL23 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL23 codon optimized
    

    Use

    Tags

    HA-Strep-Strep (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5FRTTO-codoptUL23-CStHA

    Plasmid

    #135099

    Purpose

    Codon-optimized HSV-1 UL23 with C-terminal StrepHA tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL23 codon optimized
    

    Use

    Tags

    Strep-Strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LSB-hsa-miR-7-1-3p

    Plasmid

    #103722

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-7-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-7-1-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti PA-mCit-GW

    Plasmid

    #113456

    Purpose

    Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    ProteinA-mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LSB-hsa-miR-16-5p

    Plasmid

    #103272

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-16-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-16-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LSB-hsa-miR-103a-3p

    Plasmid

    #103168

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-103a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-103a-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Plasmid

    #101236

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K918A/V922A/R925A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K918A, V922A, R925A and Q926A

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LSB-hsa-miR-6511a-3p

    Plasmid

    #103705

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-6511a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-6511a-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LSB-hsa-miR-6511a-5p

    Plasmid

    #103706

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-6511a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-6511a-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LSB-hsa-miR-181b-5p

    Plasmid

    #103278

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-181b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-181b-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti PA-NL

    Plasmid

    #113453

    Purpose

    Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-NanoLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2 Notch1 LNG (CC→SS)-6MT

    Plasmid

    #41739

    Depositor

    Raphael Kopan

    Article

    Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Promoter

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

23. TetO-FUW-Hes3

    Plasmid

    #61535

    Purpose

    Expresses Hes3 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Hes3 (Hes3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Plasmid

    #101235

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant F491A/M495A/T496A/N497A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    F491A, M495A, T496A, N497A and Q926A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Plasmid

    #101234

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant T657A/G658A/W659A/R661A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    T657A, G658A, W659A, R661A and Q926A

    Promoter

    T7

    Available Since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid

    #101220

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A and Q926A

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid

    #101232

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G528A, V583A, E584A, D585A, N588A and Q926A

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pME-Lifeact-mRuby2-3xMyc (JDW 1246)

    Plasmid

    #242554

    Purpose

    Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mRuby2
    

    Use

    Gateway subcloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pME-zFUCCI (JDW 1464)

    Plasmid

    #242558

    Purpose

    Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
    

    Use

    Gateway subcloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME-H2B-mStayGold-WPRE (JDW 1384)

    Plasmid

    #242550

    Purpose

    Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mStayGold (monomeric StayGold)
    

    Use

    Gateway subcloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge