We narrowed to 17,659 results for: URE

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pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_CS A_tTA-BFP

Plasmid

#58873

Purpose

MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1_p pET24b

Plasmid

#127852

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Tags

3C Cleavage site, His Tag, and MBP

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

Plasmid

#32735

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
Psicheck2 Sox4 WT 3'UTR oligo

Plasmid

#26990

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

Sox4 wt UTR oligo (SOX4 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

bp 449-509 of SOX4 3' UTR

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_SH3BP4-1/1

Plasmid

#91532

Purpose

Protein expression and purification of human SH3 domain construct SH3BP4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3BP4-1/1 (SH3BP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS2-1/3

Plasmid

#91387

Purpose

Protein expression and purification of human SH3 domain construct SORBS2-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS2-1/3 (SORBS2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_VAV1-1/2

Plasmid

#91355

Purpose

Protein expression and purification of human SH3 domain construct VAV1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV1-1/2 (VAV1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pOGG113

Plasmid

#133124

Purpose

pL0M-PU-NoP, promotorless region used as negative control

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

NoP promotorless region from plasmid pIJJ11268

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAM-1/1

Plasmid

#91428

Purpose

Protein expression and purification of human SH3 domain construct STAM-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAM-1/1 (STAM Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PACSIN3-1/1

Plasmid

#91335

Purpose

Protein expression and purification of human SH3 domain construct PACSIN3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN3-1/1 (PACSIN3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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M26V DJ-1 pET15b

Plasmid

#60688

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26V DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26V

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pMath3-luc

Plasmid

#51312

Purpose

Luciferase reporter driven by the Math3 promoter

Depositor

Ryoichiro Kageyama

Article

Tsuda et al J Biol Chem. 1998 Mar 13;273(11):6327-33

Insert

Math3 promoter (-2.8k- +196) (Neurod4 Mouse)

Use

Luciferase

Tags

luciferase

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2Q545AK570A

Plasmid

#14105

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Gln545 to Ala and Lys570 to Ala

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter mir129-2-wt

Plasmid

#46730

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir129-2-wt (MIR129-2 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2H4

Plasmid

#67094

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H4 (GTF2H4 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-R4R3-stop

Plasmid

#113745

Purpose

Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

stop cassette

Use

CRISPR; Gateway entry vector

Mutation

Added 3 stop codons to the original sequence; see…

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 MAPK13

Plasmid

#67077

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

MAPK13 (MAPK13 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pIS47 C20orf139 3'UTR mut

Plasmid

#14490

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

C20orf139 3'UTR mutant (SRXN1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only

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pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 Budding Yeast, A. victoria)

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Available Since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

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CCD+BATS

Plasmid

#30499

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Barkor (ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

71-180aa (CCD) + 413-492aa (BATS)

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 ATF3

Plasmid

#67057

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ATF3 (ATF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

M2I

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
PBB-79

Plasmid

#8813

Depositor

Cynthia Wolberger

Article

Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

Insert

GABPb1 (1-157) (Gabpb1 Mouse)

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2K570A

Plasmid

#14102

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys570 to Ala

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter mir30-wt

Plasmid

#46695

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir30-wt (MIR30A Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CAMK1

Plasmid

#67075

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CAMK1 (CAMK1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pQE9-His-p97deltaD2(K251A)

Plasmid

#17230

Depositor

Hemmo Meyer

Article

Ramadan et al Nature. 2007 Dec 20. 450(7173):1258-62.

Insert

p97 (Vcp Mouse)

Tags

His

Expression

Bacterial

Mutation

change codon 457 to stop codon, change lysine 25…

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter mir30-5

Plasmid

#46692

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir30-5 (MIR30A Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pBJ1 neo-mo FceRI beta

Plasmid

#8604

Depositor

Jean Kinet

Article

Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

Insert

mouse Fc epsilon RI beta cDNA (Ms4a2 Mouse)

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pCINTBeta1

Plasmid

#14067

Depositor

Richard Hynes

Article

Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

Insert

Chicken Beta 1 Integrin (ITGB1 Chicken)

Expression

Mammalian

Mutation

PstI-BglI fragment was blunt end cloned and EcoRI…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only