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Kv2.1 K+ channel scFv [L83/11]

Plasmid

#190519

Purpose

Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma L83/11.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [K89/41]

Plasmid

#190496

Purpose

Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma K89/41.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [K89/34]

Plasmid

#190495

Purpose

Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma K89/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb1 Mouse)

Tags

HA, 6xHis, Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [K89/34]

Plasmid

#190494

Purpose

Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma K89/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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KChIP2b K+ channel scFv [K60/73]

Plasmid

#190493

Purpose

Mammalian Expression of KChIP2b K+ channel scFV. Derived from hybridoma K60/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

KChIP2b K+ channel (Rattus norvegicus) recombinant scFV (Kcnip2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Kv2.2 K+ channel scFv [N372B/1]

Plasmid

#190548

Purpose

Mammalian Expression of Kv2.2 K+ channel scFV. Derived from hybridoma N372B/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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RAT +D122H

Plasmid

#187426

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Expression

Insect

Mutation

changed aspartic acid at 129 for histidine

Promoter

PH and p10

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RAT +R111E

Plasmid

#187425

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Expression

Insect

Mutation

changed arginine at 118 to glutamic acid

Promoter

PH and p10

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.337T>C-Flag

Plasmid

#169169

Purpose

Expresses EPHX1 c.337T>C in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Tags

Flag

Expression

Mammalian

Mutation

c.337T>C

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.416A>G-Flag

Plasmid

#169170

Purpose

Expresses EPHX1 c.416A>G in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Tags

flag

Expression

Mammalian

Mutation

c.337T>C

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-Entry-EPHX1c.997A>C-Flag

Plasmid

#169171

Purpose

Expresses EPHX1 c.997A>C in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Tags

Flag

Expression

Mammalian

Mutation

c.997A>C

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-Entry-EPHX1c.1212G>C-Flag

Plasmid

#169172

Purpose

Expresses EPHX1 c.1212G>C in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Tags

Flag

Expression

Mammalian

Mutation

c.1212G>C

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-Entry-EPHX1c.1288G>C-Flag

Plasmid

#169173

Purpose

Expresses EPHX1 c.1288G>C in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Tags

Flag

Expression

Mammalian

Mutation

c.1288G>C

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-ILDR2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156562

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ILDR2 (ILDR2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LEP-Fc(DAPA)-AviTag-6xHis

Plasmid

#156538

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LEP (LEP Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-CD28-Fc(DAPA)-AviTag-6xHis

Plasmid

#156525

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD28 (CD28 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-720 CTLA4-GFP R75W

Plasmid

#186125

Purpose

CTLA4 gene knockin with mutation

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

R75W

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-732pUC19-tNGFR-P2A-STIM1

Plasmid

#186119

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

STIM1 (STIM1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-719pUC19-tNGFR-P2A-CD3Z

Plasmid

#186098

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD3Z (CD247 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-456 tNGFR-P2A-ITGB1

Plasmid

#186083

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

ITGB1 (ITGB1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge