We narrowed to 18,281 results for: Met;

1. CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)

   Plasmid

   #135459

   Purpose

   Mammalian expression of SPARK protease component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   eGFP-p2A-HA-βarrestin2-uTEV1Δ
   

   Use

   Lentiviral

   Tags

   eGFP, HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV PRDM16

   Plasmid

   #15504

   Depositor

   Bruce Spiegelman

   Article

   Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

   Insert

   PRDM16 (isoform 4) (Prdm16 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES2-eGFP-Ofd1-Myc

   Plasmid

   #24560

   Depositor

   Jeremy Reiter

   Article

   Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

   Insert

   Ofd1 (Ofd1 Mouse)
   

   Use

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHIP_RNA2FLAG

   Plasmid

   #61261

   Purpose

   Flag tagged Orsay virus RNA2 genome segment expression plasmid

   Depositor

   David Wang

   Article

   Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

   Insert

   Orsay RNA2
   

   Use

   Tags

   Flag fused

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

   Plasmid

   #135458

   Purpose

   Mammalian expression of SPARK TF component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   β2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
   

   Use

   Lentiviral

   Tags

   Flag, V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUdOm6.1

   Plasmid

   #210286

   Purpose

   Empty backbone with trimethoprim resistance for high expression in mammalian cells

   Depositor

   Corrie daCosta

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMHE-NLS-mEGFP

   Plasmid

   #105527

   Purpose

   T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Nucleoplasmin
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   NLS of nucleoplasmin (encoding amino acids KRPAAT…

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

   Plasmid

   #55218

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pkk223_Null

   Plasmid

   #213111

   Purpose

   pkk223 backbone plasmid

   Depositor

   Martin Horvath

   Article

   Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCB-hDot1Lwt

    Plasmid

    #74173

    Purpose

    For packaging hDot1Lwt to retrovirus.

    Depositor

    Yi Zhang

    Article

    Okada et al Cell. 2005 Apr 22;121(2):167-78.

    Insert

    hDot1L (DOT1L Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJet1.2 kanR cassette

    Plasmid

    #117122

    Purpose

    Kanamycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Kanamycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTKDP-dhfr

    Plasmid

    #71323

    Purpose

    Donor plasmid - trimethoprim resistant donor cassette

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. UbC-(mNeonGreen)4-tDeg

    Plasmid

    #129404

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)4-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKL11-GCaMP6f bb100

    Plasmid

    #158983

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23100 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

    Plasmid

    #48294

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAH01Sp::Cas9

    Plasmid

    #128160

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH2B-miRFP680-N1

    Plasmid

    #197229

    Purpose

    Expresses the fusion protein of H2B-miRFP680 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    H2B-miRFP680
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. BASIC_SEVA_27_pKD46.10

    Plasmid

    #178922

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pQdCas12a.luxR(mut)-sggfp(C)

    Plasmid

    #236043

    Purpose

    The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. B-gTEMP/pRSET-b

    Plasmid

    #188446

    Purpose

    B-gTEMP for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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