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We narrowed to 26,060 results for: expression vector

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Showing: 6201 - 6220 of 26060 results

  9. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  14. Ths pUAST-HA

    Plasmid
    #69769
    Purpose
    Thisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    Klingseisen et al Development. 2009 Jul;136(14):2393-402.
    Insert
    Thisbe (FGF8like-1)
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA-Tag
    Expression
    Insect
    Mutation
    Encodes amino acids 1-739 of Thisbe (NP_610701.2)…
    Promoter
    hsp70 promoter
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. IBMc326

    Plasmid
    #161949
    Purpose
    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161949-plasmid-map-sequence-id-327545

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  17. pENTR-D-kcnj13

    Plasmid
    #173143
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173143-plasmid-map-sequence-id-343318

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  18. pENTR-D-kcnj1b

    Plasmid
    #173126
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj1b (kcnj1b Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173126-plasmid-map-sequence-id-342841

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  20. IBMc227

    Plasmid
    #161948
    Purpose
    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161948-plasmid-map-sequence-id-326357

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Showing: 6201 - 6220 of 26060 results