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pRRG227-Wnt1

Plasmid

#99073

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)

Depositor

Phillip Newmark

Publication

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Wnt1 (also known as WntP-1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG304-Ndk

Plasmid

#99072

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Ndk (nou darake)

Depositor

Phillip Newmark

Publication

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Ndk (nou darake) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

Plasmid

#75118

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRD7

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pET30Xa-SBL1(160S)

Plasmid

#66872

Purpose

Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

Depositor

Betty Gaffney

Publication

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)

Tags

Histag from pET30Xa-LIC

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMKO.1-puro/hFOXH1 shRNA1

Plasmid

#59303

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Publication

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Promoter

hU6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
mTagBFP2-Dectin1A-C-10

Plasmid

#55290

Purpose

Localization: Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Dectin1A (CLEC7A Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mTagBFP2-Cx32-7

Plasmid

#55287

Purpose

Localization: Gap Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cx32 (GJB1 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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cpc-PHLS (g)

Plasmid

#52311

Purpose

Replaces the cpc operon in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Publication

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Synechocystis endogenous cpc-operon promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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H2A-Bbd-MYC

Plasmid

#45172

Depositor

Brian Chadwick, Hunt Willard

Publication

Chadwick et al J Cell Biol. 2001 Jan 22;152(2):375-84.

Insert

H2AFB1 (H2AFB1 Human)

Tags

HIS and MYC

Expression

Mammalian

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3/HIP1/ΔLD

Plasmid

#31253

Depositor

Theodora Ross

Publication

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Expression

Bacterial

Mutation

Deltion of nucleotides 767-1335

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMIEG3-NFAT2

Plasmid

#40351

Depositor

Alexander Dent

Publication

Wang et al J Immunol. 2006 Jan 15;176(2):880-8.

Insert

NFAT2 (NFATC1 Mouse)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1MycHis/HIP1/ΔT

Plasmid

#31251

Depositor

Theodora Ross

Publication

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Tags

his and myc

Expression

Bacterial

Mutation

deletion of TALIN region

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKG1

Plasmid

#23413

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKG1 (PRKG1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Tag2B Flag BimEL(TD)

Plasmid

#23092

Depositor

Roger Davis

Publication

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bim EL (TD) (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Threonine 112 to Aspartate

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pET28(a)-hFX-HA

Plasmid

#14991

Depositor

Michael Ristow

Publication

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pHIV-NAT-FLAG-CIP2A FL (sg2R)

Plasmid

#222624

Purpose

Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

Depositor

Daniel Durocher

Publication

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMB1-A-TelR

Plasmid

#204979

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Publication

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-FKBP-PIP5K1C_Kina-HD

Plasmid

#202741

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTagBFP2:FKBP1A(3-108):[GGSA]4GG:PIP5K1C(79-366)(D101R/R304D) (PIP5K1C Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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hSyn-Aion-ts-citrine-ER

Plasmid

#192575

Purpose

AAV-vector for optogenetic long-term silencing of neurons

Depositor

Simon Wiegert

Publication

Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

Insert

Aion

Use

AAV

Tags

ts-Citrine-ER

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

human synapsin

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 N200Q/N219Q

Plasmid

#121454

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Publication

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N200Q/N219Q

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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