We narrowed to 11,691 results for: NSI

1. pCAGGS-PPP1CB-miRFP703

   Plasmid

   #178524

   Purpose

   Expresses PP1c-miRFP703 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   PP1c-miRFP703 (PPP1CB Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEX-GFP-hLC3WT

   Plasmid

   #24987

   Depositor

   Isei Tanida

   Article

   Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

   Insert

   a wild type human MAP1-LC3B fused with EGFP (MAP1LC3B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEX-YFP-hLC3WT

   Plasmid

   #24989

   Depositor

   Isei Tanida

   Article

   Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

   Insert

   a wild type human MAP1-LC3B fused with EYFP (MAP1LC3B Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pAc5.1-V5-HisB-ObKHC-wt-HaloTag

   Plasmid

   #201551

   Purpose

   Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Kinesin-1 (partial)
   

   Tags

   HaloTag

   Expression

   Insect

   Mutation

   Codon-optimized for Drosophila expression

   Promoter

   Ac5

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV5B+longBRD4

   Plasmid

   #157792

   Purpose

   Expression of long isoform of BRD4

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   long BRD4 (BRD4 Human)
   

   Tags

   His6

   Expression

   Insect

   Promoter

   polyhedron

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP

   Plasmid

   #232754

   Purpose

   Expresses EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   EGFP
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hsyn-GRAB_DA-mut

   Plasmid

   #140555

   Purpose

   Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   DA-insensitive sensor GRAB_DA-mut (control sensor)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TRE3G-SOX17

   Plasmid

   #104541

   Purpose

   PiggyBac vector encoding doxycycline-inducible human SOX17

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   SRY-box 17 (SOX17 Human)
   

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3G

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYDE009

   Plasmid

   #194151

   Purpose

   Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   tetR-tetP-dcas9-rrnB T1-T7Te
   

   Expression

   Bacterial

   Promoter

   tetp

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid

    #191164

    Purpose

    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP (additional cutsites)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-p38KTRmCerulean3

    Plasmid

    #59149

    Purpose

    ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
    

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Lyso-InPAkt

    Plasmid

    #184053

    Purpose

    Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Lyso-InPAkt
    

    Tags

    Lysosome-associated membrane protein 1 (LAMP1).

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TAC-OK+9MS-Cas9

    Plasmid

    #120354

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OK+9MS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF-ABEmax

    Plasmid

    #164415

    Purpose

    EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    ABEmax
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-shCTRL

    Plasmid

    #181875

    Purpose

    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    control shRNA with no known targets in the mouse genome
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human HUWE1 (3993-4374)

    Plasmid

    #110754

    Purpose

    Bacterial Expression of HUWE1 HECT domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    N-His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. tdp43-EGFP construct6

    Plasmid

    #28199

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-314 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

18. As0

    Plasmid

    #123158

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, ParsR-ABS67, PhrpLE, PrinA

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. tdp43-EGFP construct7

    Plasmid

    #28200

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-274 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

20. CDK1as_T2A_Zeo

    Plasmid

    #118596

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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