We narrowed to 10,134 results for: ada

1. qTAG-C-moxGFP-Puro-H3C2

   Plasmid

   #207783

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAAV-hSyn-DIO-GRAB_CCK1.0

   Plasmid

   #208674

   Purpose

   Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GRAB_NPYmut

   Plasmid

   #208679

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ApoE3_mCh-SspB (pBS1144)

   Plasmid

   #185326

   Purpose

   For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE3
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX307 DEDD

   Plasmid

   #117744

   Purpose

   Open reading frame vector encoding DEDD

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   DEDD1 (DEDD Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKK-NoTag

   Plasmid

   #105767

   Purpose

   Control vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Expression

   Mammalian

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-shGBP1.1.mKO2

   Plasmid

   #85209

   Purpose

   TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GBP1 (guanylate binding protein 1) (GBP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTIGER_tdTomato::3xFLAG::dCrk

   Plasmid

   #131138

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Tags

   3XFLAG and tdTomato

   Expression

   Insect

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCold I-Hero13

   Plasmid

   #187929

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero13

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero13 (BEX3 Human)
   

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-APPL1-R146A/K152A/R154A

    Plasmid

    #59767

    Purpose

    Expresses APPL1 Endosomal Localization Mutant

    Depositor

    Donna Webb

    Article

    Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

    Insert

    APPL1 (APPL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R146A/K152A/R154A

    Promoter

    CMV

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA5-FRT-T0-Flag-HROB

    Plasmid

    #135298

    Purpose

    Expresses Flag-tagged HROB in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

    Insert

    HROB (HROB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pHAGE2-TetOminiCMV-Klf4

    Plasmid

    #136613

    Purpose

    Dox-inducible lentiviral vector expressing mouse Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Blast-sTagRFP-TUBA1B

    Plasmid

    #207768

    Purpose

    Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGEX 4t1 RARalpha (1-187)

    Plasmid

    #35558

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1-187

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pKK-FLAG-TEV

    Plasmid

    #105768

    Purpose

    Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDN-G1TGt

    Plasmid

    #44508

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::yEGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. S1 1029 CP ABEMax strategy 1

    Plasmid

    #135357

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-nSpyCas9 aa 1029
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV2_hSyn_Phobos_Citrine

    Plasmid

    #98216

    Purpose

    Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSG5-FLAG-mEKLF

    Plasmid

    #67833

    Purpose

    Expression of FL-EKLF driven by SV40 promoter

    Depositor

    James Bieker

    Article

    Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    EKLF (Klf1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Full-length mEKLF is aa 20-376; amino acid 19 is …

    Promoter

    SV40

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pMKO.1-shCcnb1

    Plasmid

    #162793

    Purpose

    Ccnb1 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Ccnb1 shRNA (Ccnb1 Mouse)
    

    Use

    RNAi and Retroviral

    Promoter

    U6

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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