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pJT23_GalL_BE-PAPAP

Plasmid

#143486

Purpose

Expresses BE-PAPAP in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-PAPAP

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRPL28(K55M) SpHIS5 (pNTI666)

Plasmid

#115436

Purpose

Expresses K55M RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Publication

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

K55M; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pPKC1 (JCE575)

Plasmid

#89773

Purpose

yeast expression of PKC1

Depositor

Juan Carlos Igual

Publication

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC1 (PKC1 Budding Yeast)

Expression

Yeast

Promoter

yeast PKC

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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D5 gene in PETCON vector

Plasmid

#87626

Purpose

expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

Depositor

Sarel Fleishman

Publication

Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

Insert

Designed allosteric Zn2+ and fluorescein binding antibody scFv D5

Tags

3x(G4S) Linker and cMyc tag on backbone

Expression

Yeast

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

Plasmid

#58830

Purpose

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

Depositor

Tim Levine

Publication

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2 x Osh2 PH domain (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Lysine 307 to Glutamate and Arginine 309 …

Promoter

PHO5

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Publication

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-100

Plasmid

#47771

Purpose

expresses AmTrac-100 in yeast

Depositor

Wolf Frommer

Publication

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-100

Tags

mcpGFP

Expression

Yeast

Mutation

A141E, T464D

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-meptrac

Plasmid

#47768

Purpose

expresses MepTrac in yeast

Depositor

Wolf Frommer

Publication

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

meptrac

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-IS

Plasmid

#47766

Purpose

expresses AmTrac-IS in yeast

Depositor

Wolf Frommer

Publication

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac1;2

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-meptrac-H194E

Plasmid

#47764

Purpose

expresses MepTrac-H194E in yeast

Depositor

Wolf Frommer

Publication

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

MepTrac-H194E

Tags

mcpGFP

Expression

Yeast

Mutation

H194E

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA3

Plasmid

#46790

Purpose

To express ATPase inactive Fun30 in budding yeast

Depositor

Patrick Varga-Weisz

Publication

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Mutation

ATP-binding site mutated replaced lysin 603 by ar…

Promoter

PGAL1

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only
12URA-B-ScTop2∆CTD

Plasmid

#214924

Purpose

Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

Depositor

James Berger

Publication

Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

Insert

topoisomerase II

Tags

6x His-TEV

Expression

Yeast

Mutation

Deleted amino acids 1178-1428

Promoter

Gal 1/10

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pUDE1084

Plasmid

#168500

Purpose

expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

Depositor

Jean-Marc Daran

Publication

Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

Inserts

Fncas12a
T7RNA polymerase

Use

CRISPR

Promoter

TDH3 and TEF1

Available since

Dec. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-SUMO10-6SIM

Plasmid

#190248

Purpose

Expression SUMO10-SIM6 in yeast cells

Depositor

Liam Holt

Publication

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

10SUMO-6SIM

Expression

Yeast

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-DIPP1-HA

Plasmid

#183946

Purpose

Expresses human HA-tagged DIPP1 from yeast GPD promoter

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

DIPP1 (NUDT3 Human)

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-PRUNE-HA

Plasmid

#183945

Purpose

Expresses human HA-tagged PRUNE from yeast GPD promoter

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

PRUNE (PRUNE1 Human)

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR01-FnCpf1

Plasmid

#176529

Purpose

Nuclease Cpf1 adapted for Aspergillus species

Depositor

Kusol Pootanakit

Publication

Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

Insert

FnCpf1

Use

CRISPR; Fungal expression

Promoter

TEF1

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDRf1-SweetTrac1

Plasmid

#169083

Purpose

Expression of SweetTrac1 biosensor in yeast

Depositor

Lily S Cheung

Publication

Park et al Proc Natl Acad Sci U S A. 2022 Jan 25;11

Insert

SweetTrac1

Expression

Yeast

Promoter

PMA1

Available since

Feb. 4, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCSN074

Plasmid

#166731

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Publication

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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GGE152

Plasmid

#120752

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_8UASxpr-TEF)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P2_8UASxpr-TEF

Use

Cloning/assembly plasmid

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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