We narrowed to 11,799 results for: nsf

1. pAAV-CAG-miRFP713

   Plasmid

   #197208

   Purpose

   Expresses the protein of miRFP713 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   miRFP713
   

   Use

   AAV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

   Plasmid

   #75295

   Purpose

   An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

   Depositor

   Brandon Harvey

   Insert

   eGFP-2A-mKv1.2 (Kcna2 Synthetic, Mouse)
   

   Use

   AAV

   Promoter

   human SYN1

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPRIME-TREX-GFP-recipient

   Plasmid

   #11661

   Purpose

   3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with Tet-responsive promoter (TREX) controlling GFP expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. BAT8 (3RJW)

   Plasmid

   #28125

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   BAT8 (EHMT2 Human)
   

   Tags

   His-tag with integrated thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acid residues 913-1193.

   Available Since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. LII destination

   Plasmid

   #104529

   Purpose

   LII backbone vector

   Depositor

   Thomas Lahaye

   Article

   Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

   Type

   Empty backbone

   Use

   Ralstonia solanacearum transformation vector

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pARv-RFP

   Plasmid

   #60021

   Purpose

   pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

   Depositor

   Radislav Sedláček

   Article

   Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-AP.Adamts2.1-DIO-GCaMP8s-WPRE

   Plasmid

   #211491

   Purpose

   conditional expression of GCaMP8s under artificial promoter Adamts2

   Depositor

   Georg Keller

   Article

   O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

   Insert

   DIO-GCaMP8s
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-AP.Baz1a.1-DIO-GCaMP8s-WPRE

   Plasmid

   #211493

   Purpose

   conditional expression of GCaMP8s under artificial promoter Baz1a

   Depositor

   Georg Keller

   Article

   O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

   Insert

   DIO-GCaMP8s
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Syn:eGFP-CaM-uTEV1Δ(220-242)

   Plasmid

   #135463

   Purpose

   Neuronal expression of uFLARE protease component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   eGFP-CaM-uTEV1Δ
   

   Use

   AAV

   Tags

   eGFP, V5

   Expression

   Mammalian

   Mutation

   S219V mutation improves stability and S153N impro…

   Promoter

   Synapsin

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GGE142

    Plasmid

    #120735

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Metabolic marker for selection after transformation in Y. lipolytica (Marker_Suc2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    Marker_Suc2
    

    Use

    Cloning/assembly plasmid

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Rap-MESA-20-M-Gal4

    Plasmid

    #84554

    Purpose

    MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

    Depositor

    Joshua Leonard

    Article

    Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

    Insert

    Rap-MESA-FRB-20-M-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-emiRFP703

    Plasmid

    #197205

    Purpose

    Expresses the protein of emiRFP703 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    emiRFP703
    

    Use

    AAV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB602-mCherry-sfGFP

    Plasmid

    #213860

    Purpose

    For Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    mCherry-sfGFP
    

    Expression

    Bacterial and Plant

    Promoter

    CaMV 35S

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid

    #85398

    Purpose

    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Inserts

    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    

    Use

    AAV

    Tags

    cMyc Tag

    Expression

    Mammalian

    Mutation

    S27A

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. JGE301_pAAV-hSyn-DIO-Flag-hM3Dq-V2(2D)-ABIcs-mCherry

    Plasmid

    #107246

    Purpose

    hM3DREADD fused to ABIcs for abscisic acid mediated recruitment of PYLcs labelled proteins.

    Depositor

    Bryan Roth

    Article

    Roth et al bioRxiv 251769

    Insert

    hM3DREADD
    

    Use

    AAV

    Tags

    ABIcs, FLAG, and mCherry

    Mutation

    T to D and S to D mutations in V2-tail

    Promoter

    hSYN

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSilencer2.1-U6-T1

    Plasmid

    #190825

    Purpose

    For mammalian expression of T1, the RNA aptamer of ncOGT. Expression is driven by a U6 promoter.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    T1 (an RNA aptamer of O-GlcNAc Transferase)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5

    Plasmid

    #104842

    Purpose

    b2AR-SPARK TF component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    hLOV1-TEVcs-FLAG-GAL4-V5
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-PaIct

    Plasmid

    #111293

    Purpose

    bacterial expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    succinyl-CoA:itaconate CoA transferase
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Jan. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-hdNadV

    Plasmid

    #83362

    Purpose

    Expresses nadV from Haemophilus ducreyi in Escherichia coli

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

    Insert

    nadV
    

    Tags

    none

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. huntingtin_V2095-V3138_CbioCflag

    Plasmid

    #194764

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV and P10

    Available Since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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