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pFYF1548 EMX1

Plasmid

#47508

Purpose

human gRNA expression vector targeting EMX1

Depositor

Keith Joung

Publication

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EMX1 (EMX1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRE118

Plasmid

#43830

Depositor

Dieter Schifferli

Publication

Edwards et al Gene. 1998 Jan 30;207(2):149-57.

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb1

Expression

Bacterial

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMX-Baf155

Plasmid

#25856

Depositor

Hans Schöler

Publication

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brg1 associated factor 155 (Smarcc1 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti CMV/TO V5-LUC Puro (w549-1)

Plasmid

#19785

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFR-PATagRFP

Plasmid

#31950

Depositor

Vladislav Verkhusha

Publication

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

EGF receptor (EGFR)

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCTCON2 Aga2P-HRP

Plasmid

#73152

Purpose

Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

Depositor

Alice Ting

Publication

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Aga2P-HRP

Tags

HA tag and myc tag

Expression

Yeast

Promoter

GAL1

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mRuby3-18aa-actin

Plasmid

#74255

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to actin

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

actin

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET15b His6-FKBPF36V

Plasmid

#73180

Purpose

Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

Depositor

Thomas Wandless

Publication

Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

Insert

FKBP(F36V) (FKBP1A Human)

Tags

His 6x

Expression

Bacterial

Mutation

F36V

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pCSF107mT-GATEWAY-3'-Myc tag

Plasmid

#67617

Purpose

A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Publication

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Myc

Expression

Mammalian

Promoter

CMV and SP6

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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F-Cdk8

Plasmid

#15366

Depositor

Joan Conaway, Ronald Conaway

Publication

Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

Insert

CDK8 (CDK8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS2HA

Plasmid

#16330

Depositor

Peter Klein

Insert

HA tag

Use

Vertebrate expression

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pemiRFP670-tubulin

Plasmid

#136573

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP670-tubulin

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-cIAP1/pRK5

Plasmid

#27972

Depositor

Xiaolu Yang

Publication

Hu et al J Biol Chem. 2003 Mar 21;278(12):10055-6

Insert

CIAP1 (BIRC2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
MSCV eIF4E IRES GFP

Plasmid

#18761

Depositor

Scott Lowe

Publication

Wendel et al Nature. 2004 Mar 18. 428(6980):332-7.

Insert

eIF4E (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
VN-alpha Synuclein

Plasmid

#89470

Purpose

expresses human alpha Synuclein fused to the N-terminal part of Venus

Depositor

Bradley Hyman, Tiago Outeiro

Publication

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLJC6-2XFLAG-TMEM192

Plasmid

#104435

Purpose

Expresses 2XFLAG-TMEM192 in mammalian cells

Depositor

David Sabatini

Publication

Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

Insert

TMEM-192 (TMEM192 Human)

Use

Lentiviral

Tags

2XFLAG

Expression

Mammalian

Promoter

Ubiquitin

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHL-FcHis

Plasmid

#99846

Purpose

mammalian expression with secretive signal and FC tag

Depositor

Edith Yvonne Jones

Publication

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EPHB2 Human)

Tags

Fc_His

Expression

Mammalian

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-TrxRFP1

Plasmid

#98996

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics

Depositor

Huiwang Ai

Publication

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMAH-POLY

Plasmid

#64915

Purpose

Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

Depositor

Huiwang Ai

Publication

Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

Inserts

Polyspecific aminoacyl-tRNA Synthetase
Amber Suppression tRNA

Tags

None

Expression

Mammalian

Promoter

CMV and U6/H1

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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