We narrowed to 6,523 results for: sas

1. pTXB1-TP4

   Plasmid

   #160087

   Purpose

   Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase/APEX2 peroxidase fusion protein
   

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX4T1-GST-LC3B

   Plasmid

   #216836

   Purpose

   For expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   LC3B (Gly exposed) (MAP1LC3B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   TAC promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPO685

   Plasmid

   #195300

   Purpose

   pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade and native array with PaqCI entry site
   

   Expression

   Bacterial

   Promoter

   Arabinose inducible arapBAD

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS23: pHelper(PmcCAST)_entry_ΔTnsD

   Plasmid

   #168156

   Purpose

   Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB and TnsC)
   PmcTniQ
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL0373 (His-MBP-TniQ)

   Plasmid

   #130638

   Purpose

   Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ
   

   Use

   CRISPR; Transposon

   Tags

   His10-MBP-TEVsite on TniQ

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS21: pHelper(PmcCAST)_entry

   Plasmid

   #168154

   Purpose

   Inducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
   PmcTniQ
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN_HA-TBK1 (E696K)

   Plasmid

   #199207

   Purpose

   Used for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   E696K

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS6: pHelper(AvCAST)_entry_ΔTnsD

   Plasmid

   #168139

   Purpose

   Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMS24: pCascade(PmcCAST)_entry

   Plasmid

   #168157

   Purpose

   Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL0721 (pDonor_L1-105)

    Plasmid

    #130645

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (short L)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL0376 (His-MBP-StrepII-TniQ)

    Plasmid

    #130641

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite-StrepII on TniQ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL0715 (pDonor_R1-57)

    Plasmid

    #130644

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (short R)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASp-FMW-attB-AthHen1

    Plasmid

    #104957

    Purpose

    Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Tags

    FLAG Myc

    Expression

    Insect

    Promoter

    pTrasposase promoter

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTXB1-TP1

    Plasmid

    #160084

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein, no linker

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTXB1-TP2

    Plasmid

    #160085

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MSI1_RRM2_pGEX

    Plasmid

    #228799

    Purpose

    Recombinant expression of mouse MSI1 RRM2 with dual-affinity tag

    Depositor

    Daniel Dominguez

    Article

    Harris et al J Biol Chem. 2025 May;301(5):108435. doi

    Insert

    Musashi-1 RRM2 (Msi1 Mouse)
    

    Tags

    GST and SBP

    Expression

    Bacterial

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSI2_2xRRM_pGEX

    Plasmid

    #228800

    Purpose

    Recombinant expression of MSI2 with dual-affinity tag

    Depositor

    Daniel Dominguez

    Article

    Harris et al J Biol Chem. 2025 May;301(5):108435. doi

    Insert

    Musashi-2 (MSI2 Human)
    

    Tags

    GST and SBP

    Expression

    Bacterial

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSI2_RRM1_pGEX

    Plasmid

    #228801

    Purpose

    Recombinant expression of MSI2 RRM1 with dual-affinity tag

    Depositor

    Daniel Dominguez

    Article

    Harris et al J Biol Chem. 2025 May;301(5):108435. doi

    Insert

    Musashi-2 RRM1 (MSI2 Human)
    

    Tags

    GST and SBP

    Expression

    Bacterial

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSI2_RRM2_pGEX

    Plasmid

    #228802

    Purpose

    Recombinant expression of MSI2 RRM2 with dual-affinity tag

    Depositor

    Daniel Dominguez

    Article

    Harris et al J Biol Chem. 2025 May;301(5):108435. doi

    Insert

    Musashi-2 RRM2 (MSI2 Human)
    

    Tags

    GST and SBP

    Expression

    Bacterial

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSI1_2xRRM_pGEX_Flag_noSBP

    Plasmid

    #228796

    Purpose

    Recombinant expression of mouse MSI1 with dual-affinity tag

    Depositor

    Daniel Dominguez

    Article

    Harris et al J Biol Chem. 2025 May;301(5):108435. doi

    Insert

    Musashi-1 (Msi1 Mouse)
    

    Tags

    Flag and GST

    Expression

    Bacterial

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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