We narrowed to 9,959 results for: Met
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Plasmid#186844PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pIND4-RC-C2-M210TAG+M252V-oCNF
Plasmid#222995PurposeExpresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variantsDepositorInsertspuhA
pufL
pufM
Phe variants PylRs
Mm Pyl tRNA for oF incorporation
Tags6xHisMutationchanged Tyr M210 to amber stop codon (TAG) for am…PromoterrrnBAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free PcPTS
Plasmid#182483PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
PTS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free ClLIS1 (M)
Plasmid#182484PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
LS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (AG4TGGA)2
Plasmid#182488PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPodA11
Plasmid#162843PurposeExpression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistanceDepositorInsertProtein Designed Pyocyanin demethylase
TagsTEV-cleavable 6x-His tagExpressionBacterialMutationA53N, I73T, A87V, M99V, A129T from WT PodAPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGASdeltaN-HA
Plasmid#186843PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-HA-human cGASdeltaN
Plasmid#186852PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGASdeltaN
Plasmid#186854PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) without tags (Adamts1 Human, Synthetic)
UseRetroviralMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-scFVDnmt3A_bGHpA
Plasmid#177349PurposeAAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsmyc and scFVExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR
Plasmid#207135PurposeDNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-inDepositorInsert5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
ExpressionMammalianAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES (M)
Plasmid#182437PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorPublicationInsertsFPPS(M)
NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES
Plasmid#182482PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HA-Halo-cmyc-GRM3 C127A
Plasmid#140458PurposeMammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127ADepositorInsertGRM3 (GRM3 Human)
TagsHalo, Signal/leader sequence from influenza hemag…ExpressionMammalianMutationCysteine 127 to alanine, Threonine 174 to alanineAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)
Plasmid#174641PurposeOptogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)DepositorInsertSSPB(micro)-mVenus-ppKin14VIb(861-1321)
TagsSSPB(micro)-VenusExpressionMammalianMutationSSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…PromoterChicken beta-actinAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-365)-mVenus-SSPB(micro)
Plasmid#174635PurposeNon-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)DepositorInsertKIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
TagsmVenus-SSPB(micro)ExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…PromoterChicken beta-actinAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS-CD4-YEML-myc
Plasmid#58538PurposeExpresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.DepositorInsertCD4 (CD4 Human)
TagsIFITM3 endocytosis Tyrosine-Glutamate-Methionine-…ExpressionMammalianMutationAdded C-terminal Tyrosine-Glutamate-Methionine-Le…Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMA-RQ-PNPLA3-I148M
Plasmid#255037PurposeContains a portion of the PNPLA3 gene to allow introduction of the I148M encoding allele by gene editingDepositorInsertPatatin-like Phospholipase domain-containing 3 (PNPLA3) (PNPLA3 Human)
ExpressionMammalianMutationMutated isoleucine 148 to methionine (I148M); rec…Available sinceApril 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-human cGASdeltaN-HA
Plasmid#186897PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-GFP-human cGASdeltaN
Plasmid#186880PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal GFP fusion (Adamts1 Human, Synthetic)
UseRetroviralTagsGFPMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only