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pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA

Plasmid

#84275

Purpose

pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Promoter

CMV

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

Plasmid

#136912

Purpose

DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

pCAG-DmrC-NLS-3xFLAG-VP64

Use

CRISPR

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1

Plasmid

#182376

Purpose

Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

3xFlag
mito-mTFP1

Expression

Mammalian

Promoter

CMV promoter and SV40 promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

Plasmid

#182381

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mCherry

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

HSV TK promoter and SV40 promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xHA-Syn-smFP-flag

Plasmid

#182563

Purpose

For mouse Nav1.6 knockin with 3xHA at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xHA donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xV5-EF1-smFP-flag

Plasmid

#182562

Purpose

For mouse Nav1.6 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA

Plasmid

#84274

Purpose

pLenti vector backbone designed to express Flag-GluN2A from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Flag-GluN2A

Use

Lentiviral

Tags

Flag

Promoter

CMV

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(V303R)-IRES-puro

Plasmid

#128659

Purpose

mammalian expression of Flag-Polß(V303R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

V303R

Promoter

CMV

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

Plasmid

#174133

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

Plasmid

#174135

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo R119A K120A nsp1

Plasmid

#175428

Purpose

Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 R119A K120A

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants R119A K120A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo E36A E37A nsp1

Plasmid

#175429

Purpose

Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 E36A E37A

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants E36A E37A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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p23-NES-RanCas13b-msfGFP-NES-Flag

Plasmid

#165073

Purpose

overexpression of RanCas13b in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RanCas13b

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

Plasmid

#173167

Purpose

Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Calcineurin Like EF-Hand Protein 1 (CHP1 Human)

Use

Retroviral

Tags

3XFLAG

Mutation

Synonymous mutations at sgRNA sites, G2A, S6A

Promoter

MMLV

Available Since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND1(P287A)-Puro

Plasmid

#172641

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(P287A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND1(T286A)-Puro

Plasmid

#172642

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro

Plasmid

#172633

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND3(P284L)-Puro

Plasmid

#172617

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(P284L) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Pro284Leu

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND2(P281L)-Puro

Plasmid

#172624

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Pro281Leu

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

Plasmid

#169960

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F370G

Promoter

CMV

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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