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pSECC-sgMARK4-B

Plasmid

#138677

Purpose

Expresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 mouse (Mark4 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK3-B

Plasmid

#138673

Purpose

Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK1-E

Plasmid

#138669

Purpose

Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 mouse (Sik1 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK3-D

Plasmid

#138675

Purpose

Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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ARB366

Plasmid

#124049

Purpose

Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB367

Plasmid

#124050

Purpose

Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S12TAG

Plasmid

#107155

Purpose

MBP-rPKL construct for production and purification of rat PKL pS12

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 12 to TAG for phosphoserine insertion

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S113TAG

Plasmid

#107157

Purpose

MBP-rPKL S113TAG for production and purification of rat PKL pS113

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 113 changed to TAG for phosphoserine inser…

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A-miR-375-mutC&D

Plasmid

#53712

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site C&D (refer t…

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutE

Plasmid

#53710

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site E (refer to cita…

Available Since

July 11, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-stop-mut-miR-375-mutA-E

Plasmid

#53714

Purpose

Luciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutations

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on the stop codon of firefly luciferase …

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA&B

Plasmid

#53711

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A&B (refer t…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutD

Plasmid

#53709

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site D (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutC

Plasmid

#53708

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site C (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutB

Plasmid

#53707

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site B (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA

Plasmid

#53706

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pBa.M6PR-GFP

Plasmid

#224407

Purpose

Mammalian expression of M6PR

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

M6PR (M6PR Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-CFP

Plasmid

#173074

Purpose

Expression vector for E. coli producing target protein with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Tags

mCerulean

Expression

Bacterial

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-sGFP-Vrg4

Plasmid

#160904

Purpose

Expresses GFP-Vrg4 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

VRG4

Tags

GFP

Expression

Yeast

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B4-Leum

Plasmid

#44312

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B4-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B4-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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