We narrowed to 8,358 results for: BLI

1. PE-SUMO CRYAB

   Plasmid

   #217670

   Purpose

   For expression of SUMO tagged human alpha B crystallin

   Depositor

   Larry David

   Insert

   CRYAB (CRYAB Human)
   

   Use

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PE-SUMO CRYBB1

   Plasmid

   #217675

   Purpose

   For expression of SUMO tagged human beta B1 crystallin

   Depositor

   Larry David

   Insert

   CRYBB1 (CRYBB1 Human)
   

   Use

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6-SUMO-pET28a-EfaCas1

   Plasmid

   #112793

   Purpose

   To express enterococcus faecalis Cas1 protein in E. coli

   Depositor

   Ailong Ke

   Article

   Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

   Insert

   EfaCas1
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-69: NPM1-mTagRFP-T

   Plasmid

   #124608

   Purpose

   Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1

   Depositor

   Allen Institute for Cell Science

   Insert

   NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hs.KRAS4b G12C

   Plasmid

   #83130

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   G12C

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQTEV-RPL9

   Plasmid

   #31334

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   ribosomal protein L9 (RPL9 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLPS-hSox2-gfp

   Plasmid

   #49390

   Purpose

   mammalian expression of human Sox2 fused to EGFP

   Depositor

   Randall McKinnon

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Cre/Lox; Acceptor vector

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   TGA (stop) to CCA (Pro) at nt 1027 with respect t…

   Promoter

   CMV

   Available since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

   Plasmid

   #159912

   Purpose

   Mutagenesis of Kcnd3

   Depositor

   Larry Zweifel

   Insert

   Kcnd3 (Kcnd3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TREtight-mTagBFP2-N2cG

    Plasmid

    #192838

    Purpose

    plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Depositor

    Ian Wickersham

    Insert

    mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PTPN11 in pENTR

    Plasmid

    #216604

    Purpose

    gateway cloning donor vector containing PTPN11

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQTEV-PPP1R1A

    Plasmid

    #31331

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    protein phosphatase 1 (PPP1R1AP1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF1-mAtf4-HT-puro

    Plasmid

    #101795

    Purpose

    Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4

    Depositor

    Seiichi Oyadomari

    Insert

    Activating transcription factor 4 (Atf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hs.KRAS4b Q61H

    Plasmid

    #83140

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61H

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TCF4 in pENTR

    Plasmid

    #216569

    Purpose

    gateway cloning donor vector containing TCF4

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T1-14-3-3 gamma GST

    Plasmid

    #13280

    Depositor

    Michael Yaffe

    Article

    Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid

    #181724

    Purpose

    Galactose inducible expression of Alpha-synuclein-H50Q

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H50Q

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQTEV-PDAP1

    Plasmid

    #31586

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    PDGFA associated protein 1 (PDAP1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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