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pET28_Caspase-4_R269D

Plasmid

#214313

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

R269D

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-18_lemur

Plasmid

#214332

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-4_lemur_catalytic domain

Plasmid

#214328

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.2

Plasmid

#208303

Purpose

Expresses ABE-RA3.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.2-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, P48A, R51H, D108G, K110R, A114V, H122R, M12…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.3

Plasmid

#208304

Purpose

Expresses ABE-RA3.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.3-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, A114V…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.0

Plasmid

#208305

Purpose

Expresses ABE-RA4.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.0-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBJ016_MTB_CoChR_KGC_eGFP_ER2

Plasmid

#209277

Purpose

Ubiquitously expresses CoChR in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

CoChR_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ035_MTB_gtACR2_KGC_eGFP_ER2

Plasmid

#209278

Purpose

Ubiquitously expresses gtACR2 in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

gtACR2_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ037_MTB_FR-GECO1c

Plasmid

#209279

Purpose

Ubiquitously expresses FR-GECO1c in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

FR-GECO1c

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoR

Plasmid

#193811

Purpose

CMV overexpression of ChemoR in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR

Tags

10xHis

Expression

Mammalian

Mutation

mScarlet: D201K

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoC-CaM

Plasmid

#193813

Purpose

Protein production of ChemoC-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoC-CaM

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoY-CaM

Plasmid

#193814

Purpose

Protein production of ChemoY-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoY-CaM

Tags

10xHis

Expression

Bacterial

Mutation

Venus:A206K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoR-CaM

Plasmid

#193815

Purpose

Protein production of ChemoR-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR-CaM

Tags

10xHis

Expression

Bacterial

Mutation

mScarlet: D201K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca0.5

Plasmid

#198632

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca0.5

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca0.2

Plasmid

#198631

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca0.2

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca1

Plasmid

#198633

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca1

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-133-HiBiT

Plasmid

#198313

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-133-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-289-HiBiT

Plasmid

#198311

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-289-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-190-HiBiT

Plasmid

#198312

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-190-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnfΔpvc15

Plasmid

#198284

Purpose

Produces PVCs lacking payloads by elimination of the payload loader (pvc15)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc15

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge