We narrowed to 10,337 results for: plasmids 101

1. TetO-FUW-PLAGL1

   Plasmid

   #61542

   Purpose

   Expresses PLAGL1 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   PLAGL1 (PLAGL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.2 mir-1-1 reporter hsa-mir-122

   Plasmid

   #46673

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-122 (MIR122 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127315

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

   Plasmid

   #127312

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

   Plasmid

   #175177

   Purpose

   Cre-dependent GCaMP6s and mBeRFP expression

   Depositor

   Bernardo Sabatini

   Article

   Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   P2A-mBeRFP

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti.puro.MCV.ER.RAZ.2

   Plasmid

   #114382

   Purpose

   This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.

   Depositor

   Reety Arora, Sudhir Krishna

   Article

   Arora et al Microb Pathog. 2019 Dec;137:103778. doi:

   Insert

   MCV NCCR and tumor T antigen regions
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MCV NCCR (viral own promoter)

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

   Plasmid

   #120268

   Purpose

   Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

   Depositor

   Edward Callaway

   Article

   Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

   Insert

   EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-miniSOG-VAMP2-citrine

   Plasmid

   #50969

   Purpose

   AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Insert

   miniSOG-VAMP2-citrine (Vamp2 Mouse, Arabdopsis thaliana)
   

   Use

   AAV

   Tags

   citrine and miniSOG

   Expression

   Mutation

   Promoter

   human synapsin promoter

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1-ASAP2f

   Plasmid

   #183757

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   ASAP2f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-Lin28A-mCCHC

    Plasmid

    #51372

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    Lin28A (LIN28A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CCHC domain mutant (H147A, H169A)

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

11. PB-TAC-OKMS-Cas9

    Plasmid

    #120353

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT-9208

    Plasmid

    #124221

    Purpose

    Cloning plasmid for a self-targeting gRNA library

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

    Plasmid

    #158604

    Purpose

    Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3.1-mas-GRK2RH-Nluc L118A

    Plasmid

    #158606

    Purpose

    Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGL2-MCT2Prom0.6

    Plasmid

    #87353

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 0.6 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    0.6 kbp proximal promoter of human MCT2 (nested d…

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1_jRCaMP1b

    Plasmid

    #183758

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    jRCaMP1b
    

    Use

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCheck-DNAJA4CDS

    Plasmid

    #41849

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    DNAJA4 CDS (DNAJA4 Human)
    

    Use

    Luciferase

    Tags

    renilla luciferase

    Expression

    Mutation

    Start codon of DNAJA4 removed

    Promoter

    Available Since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pAT-9222

    Plasmid

    #124222

    Purpose

    Cloning plasmid for EGxFP assay with self-targeting gRNA-s

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA between EGFP halves
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJS278

    Plasmid

    #134139

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255, R155A

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

    Plasmid

    #127308

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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