We narrowed to 8,596 results for: Set

1. 14UAS PSD95:GFP 5UAS DSRedExpress

   Plasmid

   #74315

   Purpose

   Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

   Depositor

   Martin Meyer, Stephen Smith

   Article

   Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

   Inserts

   zebrafish PSD95
   DsRedExpress
   

   Tags

   EGFP

   Mutation

   Y599H and I793S (please see depositor comment bel…

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCT-tRNA

   Plasmid

   #133813

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
   

   Use

   CRISPR

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

   Plasmid

   #66707

   Purpose

   expresses a single gRNA and VP64-dCas9-VP64

   Depositor

   Charles Gersbach

   Inserts

   hU6-gRNA expression cassette
   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Mutation

   D10A and H840A

   Promoter

   hU6 and hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pR26 GFP Dest

   Plasmid

   #74283

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS82

   Plasmid

   #68110

   Purpose

   Integration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replication

   Depositor

   Maggie Smith

   Article

   Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

   Inserts

   aac3(IV)
   Hyg
   oriT
   attP
   int
   

   Use

   Streptomyces integrating vector

   Mutation

   Fragment

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PL552

   Plasmid

   #68407

   Purpose

   Donor empty vector with floxed puromycin resistance gene expression cassette

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   mouse PGK promoter

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MXS_CMV::rtTA3-bGHpA

   Plasmid

   #62445

   Purpose

   MXS_chaining vector with CMV::rtTA3-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the reverse tetracycline transactivator with CMV promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGSHU

   Plasmid

   #72244

   Purpose

   Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

   Depositor

   Michael Resnick, Francesca Storici

   Article

   Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

   Inserts

   KlURA3
   hyg
   I-SceI
   

   Expression

   Bacterial

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC-HA-Nedd8-P2A-Hygro

   Plasmid

   #124306

   Purpose

   Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   Nedd8 (NEDD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATM-Halo HRD

    Plasmid

    #207082

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    None

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGolden-Neo

    Plasmid

    #51422

    Purpose

    Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEED-LoxP-sfGFP:ALFA

    Plasmid

    #219660

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP:ALFA SEED Cassette (LoxP)
    

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. JC201: pMVP/Ad/5-35-DEST

    Plasmid

    #121847

    Purpose

    pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMA-SpCas9-g3

    Plasmid

    #80786

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBlu/gRNA

    Plasmid

    #59188

    Purpose

    Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Guide RNA Cassette
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6 (Arabidopis)

    Available Since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Level 1 P2 OsUbiP:Cas9:NosT

    Plasmid

    #165424

    Purpose

    GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    

    Use

    Synthetic Biology

    Promoter

    Os Ubiquitin promoter :: Cas9 :: NosTerminator

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-3HA-kanMX6

    Plasmid

    #39295

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    3HA, A. gossypii translation elongation factor 1a…

    Available Since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC5

    Plasmid

    #118863

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJ204-loxP-100RPU.2-coEPO-lox2272

    Plasmid

    #154828

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    coEPO
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    Codon-optimized (Cricetulus griseus)

    Promoter

    100RPU.2

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC020 - LseCas13a from Listeria seeligeri

    Plasmid

    #91910

    Purpose

    Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LseCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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