We narrowed to 16,043 results for: GRN

1. pTC441

   Plasmid

   #91218

   Purpose

   protoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNAs targeting MLO gene in barley
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTC437

   Plasmid

   #91219

   Purpose

   protoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNAs targeting MLO gene in barley
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTC366

   Plasmid

   #91215

   Purpose

   protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   2 gRNAs targeting tomato ARF8A
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP2

   Plasmid

   #79874

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into amyE

   Depositor

   Carol Gross, Jason Peters, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   veg

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDG458

   Plasmid

   #100900

   Purpose

   SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP3

   Plasmid

   #79875

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into thrC

   Depositor

   Carol Gross, Jason Peters, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   veg

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ141-ZmUbi-RZ-Lb

   Plasmid

   #86197

   Purpose

   LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   gRNA cloning site
   

   Use

   CRISPR

   Promoter

   Maize ubiquitin 1

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDECKO-mCherry GFP

   Plasmid

   #78535

   Purpose

   Control plasmid (paired gRNAs against GFP)

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Direct-seq_lentiGuide-Puro-Loop2-8A8G

   Plasmid

   #157983

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid

    #157981

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPTG-GG-sg2+sg3

    Plasmid

    #127199

    Purpose

    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA
    

    Use

    Empty sgrna plasmid

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROPseq-Guide-Puro

    Plasmid

    #86708

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Christoph Bock

    Article

    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDG330

    Plasmid

    #100898

    Purpose

    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PDG458 V3

    Plasmid

    #226959

    Purpose

    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458 V3

    Plasmid

    #226957

    Purpose

    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJR107

    Plasmid

    #187245

    Purpose

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJR106

    Plasmid

    #187244

    Purpose

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDG335

    Plasmid

    #100899

    Purpose

    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

    Plasmid

    #157986

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge