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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,201 results for: mme

Showing: 6361 - 6380 of 7201 results

  4. CIBN-CAAX

    Plasmid
    #79574
    Purpose
    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    CIBN (CIB1 Mustard Weed)
    Tags
    C-terminal CAAX-box
    Expression
    Mammalian
    Mutation
    K93A, R94A, K106A, K107A (please see depositor co…
    Promoter
    CMV
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79574-plasmid-map-sequence-id-314173

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  5. pM-ABE

    Plasmid
    #196292
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding ABE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196292-plasmid-map-sequence-id-390025

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  7. pM-Cas9

    Plasmid
    #196325
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196325-plasmid-map-sequence-id-390015

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  8. LSB-hsa-let-7a-5p

    Plasmid
    #103146
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7a-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103146-plasmid-map-sequence-id-213119

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  9. pM-Cas12a

    Plasmid
    #196291
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    Use
    CRISPR
    Tags
    Flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196291-plasmid-map-sequence-id-389824

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  10. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65206-plasmid-map-sequence-id-490956

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  11. LSB-hsa-let-7f-5p

    Plasmid
    #103156
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7f-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103156-plasmid-map-sequence-id-213245

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  13. pAnc80L65

    Plasmid
    #68837
    Purpose
    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details
    Depositor
    Luk Vandenberghe
    Article
    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1
    Insert
    Ancestral AAV Capsid Anc80L65
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    Rep2
    Available Since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    68837-plasmid-map-sequence-id-121219

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  14. MICU-APEX2

    Plasmid
    #79057
    Purpose
    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    C-terminal APEX2 fused MICU1 (MICU1 Human, Soybean)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Sept. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79057-plasmid-map-sequence-id-147904

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  16. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid
    #89873
    Purpose
    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production
    Depositor
    Hyungbae Kwon
    Article
    Lee et al Nat Methods. 2017 May;14(5):495-503. doi
    Insert
    bArrestin2-TevC-P2A-TdTomato
    Use
    AAV
    Tags
    TdTomato
    Expression
    Mammalian
    Mutation
    R173H in TdTomato (Please see depositor comments …
    Promoter
    Human synapsin
    Available Since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    89873-plasmid-map-sequence-id-177108

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Showing: 6361 - 6380 of 7201 results