We narrowed to 5,049 results for: golden gate
-
Plasmid#234184PurposeHeterologous protein expression of oxysterol-binding protein homolog 1 in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits
-
pAIP4_4RZ9
Plasmid#234164PurposeHeterologous protein expression of pre-mRNA-splicing factor 38A in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5YPV
Plasmid#234150PurposeHeterologous protein expression of malonyl CoA-acyl carrier protein transacylase in Escherichia coliDepositorInsert5YPV
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1H6T
Plasmid#234146PurposeHeterologous protein expression of internalin B (InlB) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1B42-pGATA23-Bxb1-SAUR-tUBQ10
Plasmid#219705PurposeLevel 1 pGATA23-Bxb1-SAUR-tUBQ10, for lateral root specific expression of Bxb1 integrase with SAUR tagDepositorInsertpGATA23:Bxb1:SAUR:tUBQ10
ExpressionPlantAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-kan
Plasmid#222827PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-kan5A
Plasmid#222830PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T11 Circuit 2 GG4-PhiC31B-Bxb1B-GG6
Plasmid#219694PurposeLevel 0 to construct history-dependent target T2BPDepositorInsertGG4-PhiC31B-Bxb1B-GG6
UseSynthetic BiologyAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T12 GG2-ER-tagBFP-tUBQ10-GG3
Plasmid#219695PurposeLevel 0 to construct history-dependent targetDepositorInsertGG2-ER-tagBFP-tUBQ10-GG3
UseSynthetic BiologyAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T13 GG3-tRBCS-mCherry-NLS-GG4
Plasmid#219696PurposeLevel 0 to construct history-dependent targetDepositorInsertGG3-tRBCS-mCherry-NLS-GG4
UseSynthetic BiologyAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1P137 - pAHP6-PhiC31-NLS-tUBQ1
Plasmid#219702PurposeLevel 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integraseDepositorInsertpAHP6:PhiC31-NLS:tUBQ1
ExpressionPlantAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1
Plasmid#219697PurposeLevel 0 to construct history-dependent targetDepositorInsertGG0-tUBQ1-NLS-NeonGreen-GG1
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T10 Circuit 1 GG4-Bxb1B-PhiC31B-GG6
Plasmid#219693PurposeLevel 0 to construct history-dependent target T2PBDepositorInsertGG4-Bxb1B-PhiC31B-GG6
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK2-J11
Plasmid#218224PurposeTerminates AtU6-promoter driven sgRNA array with two guides, MoClo CompatibleDepositorInsertlinker sequence
UseCRISPRAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK2-K3
Plasmid#218221PurposeFuses Guides 1+2 to guides 3+4 in an of AtU6-promter driven sgRNAsDepositorInsertlinker sequence
UseCRISPRAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK4-J11
Plasmid#218225PurposeTerminates AtU6-promoter driven sgRNA array with four guides, MoClo CompatibleDepositorInsertlinker sequence
UseCRISPRAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK6-J11
Plasmid#218226PurposeTerminates AtU6-promoter driven sgRNA array with tsix guides, MoClo CompatibleDepositorInsertlinker sequence
UseCRISPRAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK4-K5
Plasmid#218222PurposeFuses Guides 3+4 to guides 6+6 in an of AtU6-promter driven sgRNAsDepositorInsertlinker sequence
UseCRISPRAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK8-J11
Plasmid#218227PurposeTerminates AtU6-promoter driven sgRNA array with eight guides, MoClo CompatibleDepositorInsertlinker sequence
UseCRISPRAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK6-K7
Plasmid#218223PurposeFuses Guides 5+6 to guides 7+8 in an of AtU6-promter driven sgRNAsDepositorInsertlinker sequence
UseCRISPRAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_161 [TermB]
Plasmid#210683PurposeLevel 0 partDepositorInsertTermB
UseSynthetic BiologyAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_160 [ermB]
Plasmid#210682PurposeLevel 0 partDepositorInsertermB
UseSynthetic BiologyAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_159 [PermB]
Plasmid#210681PurposeLevel 0 partDepositorInsertPermB
UseSynthetic BiologyAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_158 [TcatP]
Plasmid#210680PurposeLevel 0 partDepositorInsertTcatP
UseSynthetic BiologyAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_157 [catP]
Plasmid#210679PurposeLevel 0 partDepositorInsertcatP
UseSynthetic BiologyAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_156 [PcatP]
Plasmid#210678PurposeLevel 0 partDepositorInsertPcatP
UseSynthetic BiologyAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAH46 - MosTI [unc-119(partial) | MCS | gfp]
Plasmid#200366PurposeCloning vector for mosTI(unc-119, gfp). MCS or Golden Gate.DepositorTypeEmpty backboneExpressionWormAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH45 - MosTI [unc-119(partial) | MCS | wrmScarlet]
Plasmid#200365PurposeCloning vector for mosTI(unc-119, wrmScarlet). MCS or Golden Gate.DepositorTypeEmpty backboneExpressionWormAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD719 DsRed-Express2-ZF173
Plasmid#138811PurposeConstitutive expression of DsRed-Express2-ZF173 under the CMV promoter.DepositorInsertDsRed-Express2-ZF173
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD718 DsRed-Express2-ZF172
Plasmid#138810PurposeConstitutive expression of DsRed-Express2-ZF172 under the CMV promoter.DepositorInsertDsRed-Express2-ZF172
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD717 DsRed-Express2-ZF151
Plasmid#138808PurposeConstitutive expression of DsRed-Express2-ZF151 under the CMV promoter.DepositorInsertDsRed-Express2-ZF151
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD707 DsRed-Express2-ZF14
Plasmid#138794PurposeConstitutive expression of DsRed-Express2-ZF14 under the CMV promoter.DepositorInsertDsRed-Express2-ZF14
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD709 DsRed-Express2-ZF36
Plasmid#138796PurposeConstitutive expression of DsRed-Express2-ZF36 under the CMV promoter.DepositorInsertDsRed-Express2-ZF36
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD710 DsRed-Express2-ZF42
Plasmid#138798PurposeConstitutive expression of DsRed-Express2-ZF42 under the CMV promoter.DepositorInsertDsRed-Express2-ZF42
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD711 DsRed-Express2-ZF54
Plasmid#138800PurposeConstitutive expression of DsRed-Express2-ZF54 under the CMV promoter.DepositorInsertDsRed-Express2-ZF54
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD712 DsRed-Express2-ZF55
Plasmid#138801PurposeConstitutive expression of DsRed-Express2-ZF55 under the CMV promoter.DepositorInsertDsRed-Express2-ZF55
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD713 DsRed-Express2-ZF62
Plasmid#138802PurposeConstitutive expression of DsRed-Express2-ZF62 under the CMV promoter.DepositorInsertDsRed-Express2-ZF62
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD160 DsRed-Express2-ZFi92
Plasmid#138803PurposeConstitutive expression of DsRed-Express2-ZFi92 under the CMV promoter.DepositorInsertDsRed-Express2-ZFi92
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD714 DsRed-Express2-ZF93
Plasmid#138804PurposeConstitutive expression of DsRed-Express2-ZF93 under the CMV promoter.DepositorInsertDsRed-Express2-ZF93
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD260 DsRed-Express2-ZFi97
Plasmid#138805PurposeConstitutive expression of DsRed-Express2-ZFi97 under the CMV promoter.DepositorInsertDsRed-Express2-ZFi97
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD715 DsRed-Express2-ZF129
Plasmid#138806PurposeConstitutive expression of DsRed-Express2-ZF129 under the CMV promoter.DepositorInsertDsRed-Express2-ZF129
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD716 DsRed-Express2-ZF150
Plasmid#138807PurposeConstitutive expression of DsRed-Express2-ZF150 under the CMV promoter.DepositorInsertDsRed-Express2-ZF150
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
T2m
Plasmid#121029PurposeMoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertECK120033736 (164.6x Voigt terminator), short attachment
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7m
Plasmid#121030PurposeMoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertS. pyogenes tracrRNA terminator
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T13m
Plasmid#121032PurposeMoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertECK120033737 (second strongest natural Voigt terminator)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C105m
Plasmid#121008PurposeMoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-AAV ssrA (has bbsI)
UseSynthetic BiologyTagsAAV degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C106m
Plasmid#121009PurposeMoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-DAS ssrA (has bbsI)
UseSynthetic BiologyTagsDAS degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C109m
Plasmid#121011PurposeMoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.DepositorInsertmfLon protease with pdt3 tag
UseSynthetic BiologyTagspdt3 mfLon degradation tagAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C70m
Plasmid#120982PurposeMoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).DepositorInsertRpaR Adam Meyer
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C41m
Plasmid#120974PurposeMoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.DepositorInsertdCas9
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only