We narrowed to 1,206 results for: SEF;

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BiP-mGFP

Plasmid

#62231

Purpose

Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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BiP-mCherry

Plasmid

#62233

Purpose

Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mCherry-KDEL

Tags

mCherry

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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myc-GABAAR Gamma2

Plasmid

#127619

Purpose

N-terminal myc-tagged Gamma2 subunit of GABAAR

Depositor

Josef Kittler

Article

Arancibia-Carcamo et al Proc Natl Acad Sci U S A. 2009 Oct 13;10

Insert

GABA-A receptor subunit gamma-2

Tags

myc

Expression

Mammalian

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKB817 (pBra-Hfq)

Plasmid

#174661

Purpose

Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

Depositor

Katherine Berry, Ann Hochschild

Article

Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

Insert

Hfq

Tags

alpha NTD

Expression

Bacterial

Promoter

lpp/lacUV5 (tandem promoter)

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pIIS18-BsaI

Plasmid

#128649

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pmRFP-SUMO1 GG

Plasmid

#13990

Depositor

Jonathan Chernoff

Article

Dadke et al Nat Cell Biol. 2007 Jan . 9(1):80-85.

Insert

SUMO1 GG (Sumo1 Mouse)

Tags

mRFP

Expression

Mammalian

Mutation

C-terminal truncation. Cannot ligate to substrate…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
Rix-PGK-Tom-W

Plasmid

#25813

Depositor

Jozsef Kiss, Patrick Salmon

Article

Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

Insert

Rix-PGK-Tom-W

Use

Lentiviral

Mutation

tdTomato from Tsien lab

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pJOE6090.1

Plasmid

#135080

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

Insert

P-rhaP-BAD-Strep-tag II-eGFP

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDH3

Plasmid

#83789

Purpose

Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Tags

CFP

Expression

Yeast

Mutation

F64L, Y66W, N146I, M153T, V163A, N164H mutations …

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pIIS18-SapI

Plasmid

#128599

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pIIS18-BsmBI

Plasmid

#128648

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27 RNAi-resistant

Plasmid

#163620

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 RNAi-resistant (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

RNAi resistance

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX302_circSMAD2_OE

Plasmid

#215238

Purpose

Overexpression of pLEX302_circSMAD2(2,7)_OE expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circSMAD2

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_LacZ

Plasmid

#215217

Purpose

Supression of shLacZ expression (Negative control)

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

LacZ shRNA

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIIS18-BufAI-2

Plasmid

#128651

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pIIS18-BufAI-1

Plasmid

#128650

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA-puro

Plasmid

#180426

Purpose

Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

gRNAscr

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag Yap1

Plasmid

#18881

Depositor

Yosef Shaul

Article

Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

Insert

Yap1 (YAP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only

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