We narrowed to 8,438 results for: app

1. eSpCas9(1.1)_No_FLAG_AAVS1_T2

   Plasmid

   #79888

   Purpose

   Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

   Plasmid

   #68375

   Purpose

   Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and TALEN; Zfn

   Tags

   3xFLAG_Twin_Strep

   Expression

   Mammalian

   Mutation

   Promoter

   human PGK1

   Available since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. IKK-2 WT

   Plasmid

   #11103

   Depositor

   Anjana Rao

   Article

   Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

   Insert

   IKK-2 (IKBKB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBSV2G_P0826-BB0323-mCherryBb

   Plasmid

   #118244

   Purpose

   Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   BB0323-mCherry translational fusion under the control of the bb0826 promoter
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-NG-PE2-BSD

   Plasmid

   #176933

   Purpose

   Expresses PE2-NG variant in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

   Insert

   PE2-NG variant
   

   Use

   Lentiviral

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PDK1-FLAG-LOV*

   Plasmid

   #202205

   Purpose

   Expresses the photoproximity labeling catalyst LOV* fused to PDK1 in mammalian cells

   Depositor

   Thomas Muir

   Article

   Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1 Human)
   

   Use

   Tags

   FLAG and LOV*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GalT-moxDendra2

   Plasmid

   #89789

   Purpose

   mammalian expression of GC localized moxDendra2

   Depositor

   Erik Snapp

   Article

   Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

   Insert

   GalT signal anchor transmembrane domain (B4GALT1 Human)
   

   Use

   Tags

   moxDendra2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPSP6+t

   Plasmid

   #48149

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-EF1a-MTS-FLAG-LOV*

   Plasmid

   #202208

   Purpose

   Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

   Depositor

   Thomas Muir

   Article

   Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
   

   Use

   Lentiviral

   Tags

   FLAG and LOV*

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPSP6

    Plasmid

    #48150

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPK1E-1+t

    Plasmid

    #48147

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPK1E-2+t

    Plasmid

    #48148

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ZE46 DeltaPGB Dvl2 pCS2P+

    Plasmid

    #17087

    Depositor

    Randall Moon

    Article

    Waxman et al Development. 2004 Dec. 131(23):5909-21.

    Insert

    Dvl-2 (dvl2 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    DeltaPGB (deletes amino acids 231-252)

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pPK1E-2

    Plasmid

    #48146

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPK1E-1

    Plasmid

    #48145

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IKK-2 K44M

    Plasmid

    #11104

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K44M, kinase inactive

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUW-tetO-NFKB1

    Plasmid

    #139822

    Purpose

    doxycycline-inducible expression of Human NFKB1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. ECFP-IKB

    Plasmid

    #111193

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

    Insert

    IKB-alpha
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6-pegRNA-H1-nick sgRNA-mCherry

    Plasmid

    #176901

    Purpose

    Expresses prime editing guide RNA and guide RNA in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Inserts

    mCherry
    gRNA scaffold
    gRNA scaffold
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, H1, and U6

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pK1E-RNAP

    Plasmid

    #48144

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase K1E
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge