159,557 results

1. AAVS1-Puro cTnT FUCCI

   Plasmid

   #136935

   Purpose

   donor plasmid for cardiac-specific FUCCI expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   Troponin T promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cTnT

   Available since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCVpuro-Cas9-EGFP

   Plasmid

   #159126

   Purpose

   Expresses active Cas9 fused with EGFP for fluorescence microscopy

   Depositor

   Taekjip Ha

   Article

   Liu et al Science. 2020 Jun 12;368(6496):1265-1269

   Insert

   Cas9 fused to EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR promoter

   Available since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

   Plasmid

   #204359

   Purpose

   AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

   Depositor

   Masao Doi

   Article

   Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

   Insert

   miniDq-mCherry-P2A-HA-KORD
   

   Use

   AAV

   Tags

   HA (for KORD) and mCherry (for miniDq)

   Expression

   Mutation

   For miniDq, the third intracellular loop (ICL3) o…

   Promoter

   hSyn

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mNeonGreen-KDEL

   Plasmid

   #226568

   Purpose

   Encodes mNeonGreen with KDEL ER lumenal target sequence

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   KDEL (ER lumenal target sequence)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-AncBE4max

   Plasmid

   #174164

   Purpose

   This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.

   Depositor

   Wensheng Wei

   Article

   Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

   Insert

   AncBE4max
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TCARZ-CBh-GFP-SV40PA-GF3-R4A22

   Plasmid

   #203767

   Purpose

   For production of template RNA for use in PRINT, GFP under CBh promoter

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   PRINT template RNA for GFP
   

   Use

   Ivt template

   Tags

   Expression

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mKeima-Red-N1

   Plasmid

   #54597

   Purpose

   Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mKeima-Red

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. 7xSMAD_RE::FLuc

   Plasmid

   #124531

   Purpose

   SMAD2 FLuc luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   SMAD FLuc reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MLM3636

    Plasmid

    #43860

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

    Depositor

    Keith Joung

    Insert

    None
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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