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pMOD_A4800EC

Plasmid

#176873

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_B4800EC

Plasmid

#176874

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pK-eCFP

Plasmid

#176559

Purpose

Expression of eCFP for auxotrophic selection in the absence of histidine

Depositor

Markus Ralser

Article

Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

Insert

eCFP

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDH006

Plasmid

#157641

Purpose

sMHT-113 fused SYNZIP18 only

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)
SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH008

Plasmid

#157643

Purpose

sMHT-113 fused to CheY only

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)
CheY-sMHT(1-114)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF035

Plasmid

#157632

Purpose

sMHT-67

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-67)-SYNZIP17
SYNZIP18-sMHT(68-230)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF044

Plasmid

#157637

Purpose

sMHT-113 F2 only

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P3S

Plasmid

#139796

Purpose

expression of biosynthetic enzyme 4CL in fusion with CC segment P3S

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

4CL:P3S

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCC2

Plasmid

#128283

Purpose

asymmetric plasmid partitioning E. coli - negative control

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, _parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV500

Plasmid

#110722

Purpose

Lentiviral system for reduced ectopic expression.

Depositor

Kevin Dean, Reto Fiolka

Article

Dean et al Biophys J. 2016 Mar 29;110(6):1456-65. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHL 2147

Plasmid

#69790

Purpose

KanR with FRT sites + pT7::LacO::SgrS::T1 terminator + ColE

Depositor

Han Lim

Article

Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

Insert

SgrS

Use

Synthetic Biology

Tags

LacO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only
PpPchE

Plasmid

#242092

Purpose

Nonribosomal peptide synthetase from Pseudomonas protegens for the production of enantiopyochelin

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

PpPchE

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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SvPchE

Plasmid

#242093

Purpose

Nonribosomal peptide synthetase from Streptomyces venezuluelae for the production of watasemysin

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

SvPchE

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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BsSrfD

Plasmid

#242095

Purpose

Thioesterase from Bacillus subtilis

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

BsSrfD

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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PaPchE-AE

Plasmid

#242103

Purpose

Adenylase-epimerase didomain of the nonribosomal peptide synthetase PchE from Pseudomonas aeruginosa for the production of pyochelin

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

PaPchE-AE

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pReceiver

Plasmid

#228594

Purpose

Mammalian expression of a single fluorescent protein, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMulti

Plasmid

#228613

Purpose

Mammalian expression of a single fluorescent protein and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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His8OphMA-21mer.

Plasmid

#189670

Purpose

His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMATEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::ECK120015170

Plasmid

#171879

Purpose

Terminator ECK120015170 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Terminator ECK120015170

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDH007

Plasmid

#157642

Purpose

sMHT-113 fused to CheA and CheY

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113) -CheA
CheY-sMHT(114-23)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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