We narrowed to 11,692 results for: NSI

1. pMSCV-GSDMD_C192A-Myc

   Plasmid

   #201168

   Purpose

   Expression of GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Mutation

   C192A

   Promoter

   MESV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABEpuro-ARAF_S214C

   Plasmid

   #51077

   Purpose

   mammalian expression of ARAF S214C

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S214C

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGB2alpha2 35s:hCas9:tNos (GB0639)

   Plasmid

   #68222

   Purpose

   Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   hCas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. DsRed +9e

   Plasmid

   #191766

   Purpose

   Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Tags

   N terminal fusion of positively charged polypetod…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nanog-CreER targeting construct

   Plasmid

   #59720

   Purpose

   Cre-ER knockin targeting construct for mouse endogenous Nanog locus

   Depositor

   Jacob Hanna

   Article

   Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

   Insert

   creERT2
   

   Use

   Mouse Targeting

   Promoter

   mNanog

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_SORBS1-1/3

   Plasmid

   #91547

   Purpose

   Protein expression and purification of human SH3 domain construct SORBS1-1/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SORBS1-1/3 (SORBS1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_SORBS1-3/3

   Plasmid

   #91553

   Purpose

   Protein expression and purification of human SH3 domain construct SORBS1-3/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SORBS1-3/3 (SORBS1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_SORBS1-2/3

   Plasmid

   #91530

   Purpose

   Protein expression and purification of human SH3 domain construct SORBS1-2/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SORBS1-2/3 (SORBS1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC_N_BET1

   Plasmid

   #111683

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   BET1 (BET1 Human)
   

   Expression

   Mammalian

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPQD1CB0107

    Plasmid

    #177040

    Purpose

    MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC_N_FBRL

    Plasmid

    #111686

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    FBRL (FBL Human)
    

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T-1-3xMyc-ERK2(K52R)

    Plasmid

    #129228

    Purpose

    Encodes Myc tagged human ERK2(K52R) for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    ERK2(K52R) (Mapk1 Rat)
    

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    Changed Lysine 52 to Arginine

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmalc4x_E323K_TEV

    Plasmid

    #47699

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed glutamic acid 323 to lysine

    Promoter

    Ptac

    Available Since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA FLAG mTOR ST

    Plasmid

    #22999

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    mTOR (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pEJS542-pCSDest2-AcrIIC5Smu

    Plasmid

    #113435

    Purpose

    Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry

    Plasmid

    #75133

    Purpose

    Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1116
    

    Use

    Retroviral

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pp32ΔCT

    Plasmid

    #67241

    Purpose

    pp32(1-149) cloned into pProEX HTb

    Depositor

    Cynthia Wolberger

    Article

    Huyton et al Protein Sci. 2007 Jul;16(7):1308-15. Epu

    Insert

    pp32 (ANP32A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-149

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pEX-CFP-hLC3ΔG

    Plasmid

    #24986

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a mutant human MAP1-LC3B fused with ECFP (MAP1LC3B Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    deleted Gly at 121

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA GAL4 CREB 1-341 M1

    Plasmid

    #22973

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

    Insert

    CREB (Creb1 Rat, GAL4-fusion)
    

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine of CREB

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSHDY*in_PpetE:BCD2:mVenus

    Plasmid

    #133970

    Purpose

    Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

    Depositor

    Pia Lindberg

    Article

    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

    Insert

    mVenus
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    inserted gene is codon optimized for Synechocyst…

    Promoter

    PpetE:BCD2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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