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pEGFP-Bik

Plasmid

#10952

Depositor

Bingliang Fang

Publication

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

human Bik gene (BIK Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET26b-SET

Plasmid

#8822

Depositor

Judy Lieberman

Publication

Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

Insert

SET (SET Human)

Tags

6XHis

Expression

Bacterial

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
GPER-Tango

Plasmid

#66299

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPER (GPER1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Sirius/pcDNA3

Plasmid

#51957

Purpose

mammalian expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Publication

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Insert

Sirius

Expression

Mammalian

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDDYFP-B

Plasmid

#40289

Depositor

Robert Campbell

Publication

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

ddYFP-B

Tags

His

Expression

Bacterial

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
AAV pCAG-FLEX-mScarlet-WPRE

Plasmid

#99280

Purpose

Can be used to generate AAV virus that will express mScarlet in the presence of Cre

Depositor

Rylan Larsen

Insert

mScarlet

Use

AAV and Cre/Lox

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMTS_mScarlet_N1

Plasmid

#85057

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

MTS (COX8A Human)

Tags

mScarlet

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTY-EF1a-wild type mKdm2b-Flag

Plasmid

#61739

Purpose

Express wild-type mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Publication

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3-myc3-L11

Plasmid

#20936

Depositor

Yue Xiong

Publication

Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

Insert

Ribosomal protein L11 (RPL11 Human)

Tags

myc3

Expression

Mammalian

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
882 pSG5L HA PTEN G129E

Plasmid

#10746

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

G129E

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

Plasmid

#32627

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Donor + Sce BFP

Use

Lentiviral

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pENTR4-GST 6P-1 (w487-1)

Plasmid

#17741

Purpose

Gateway entry vector for N-term GST fusion, frame 6P-1

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

GST

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRDA_221

Plasmid

#169142

Purpose

Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

Depositor

John Doench, David Root

Insert

EGFP , EGFP sgRNA

Use

CRISPR and Lentiviral

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HA-GFP

Plasmid

#137763

Purpose

Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Publication

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSkylan-NS-N1

Plasmid

#86785

Purpose

for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

Depositor

Pingyong Xu

Publication

Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

Type

Empty backbone

Tags

Skylan-NS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAVS1-DRGFP

Plasmid

#113193

Purpose

DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

Depositor

Adam Karpf

Insert

DR-GFP

Use

Donor construct with t2a-hygromycin for gene trap…

Expression

Mammalian

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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alpha catenin conformation sensor

Plasmid

#71709

Purpose

This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

Depositor

Deborah Leckband

Publication

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin FRET Sensor

Tags

ECFP and YPET

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag C/EBPb LAP

Plasmid

#66979

Purpose

Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Publication

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP beta (Cebpb Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII-Jaws-KGC-GFP-ER2

Plasmid

#65015

Purpose

AAV-mediated expression of Jaws under the CaMKII promoter

Depositor

Edward Boyden

Publication

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-C3-Sec15

Plasmid

#53760

Purpose

pEGFP-C3 plasmid that expresses Sec15

Depositor

Channing Der

Publication

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec15 (EXOC6 Human)

Tags

GFP

Expression

Mammalian

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article