We narrowed to 10,632 results for: t7

1. pET28b_TypeIC_acrIC4_invivo

   Plasmid

   #196403

   Purpose

   expresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

   Depositor

   David Taylor

   Article

   O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

   Insert

   anti-CRISPR IC4 protein
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSrtA-MT4

   Plasmid

   #200306

   Purpose

   Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

   Depositor

   Artur Krężel

   Article

   Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

   Insert

   eSrtA-LPETG-MT4 (MT4 Human)
   

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSrtA-MT3

   Plasmid

   #200305

   Purpose

   Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

   Depositor

   Artur Krężel

   Article

   Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

   Insert

   eSrtA-LPETG-MT3 (MT3 Human)
   

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 AMSHLP 16-218

   Plasmid

   #180609

   Purpose

   Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   AMSHLP residues 16-218
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 16-218

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1_SUL-I

   Plasmid

   #200172

   Purpose

   Amino acids 25-308 of SUL-I with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

   Depositor

   Michael Jewett

   Article

   Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

   Insert

   SUL-I
   

   Tags

   6x His tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. NeuA.pJL1

   Plasmid

   #199115

   Purpose

   In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)

   Depositor

   Michael Jewett

   Article

   Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

   Insert

   N-acylneuraminate cytodiylyltransfease (NeuA)
   

   Tags

   Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Derp2.pJL1

   Plasmid

   #199116

   Purpose

   In vitro expression of Der p 2, a major common allergen from Dermatophagoides pteronyssinus (dust mite), with synthetic site for glycosylation encoded (GGNWTT)

   Depositor

   Michael Jewett

   Article

   Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

   Insert

   Der p 2 dust mite allergen
   

   Tags

   Glyctag, His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p32m3C Trx-CN

   Plasmid

   #196474

   Purpose

   Protein expression of Thiolredoxin-3C cleavage site-CarHCN

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   Trx-CN
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE80l AEC

   Plasmid

   #196473

   Purpose

   Protein expression of SpyTag-ELP-CarHC

   Depositor

   Fei Sun

   Article

   Yang et al Matter (2020) 2, 233–249

   Insert

   AEC
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLS.539

    Plasmid

    #184958

    Purpose

    Test effect of a1/a2 length on editing fabH using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
    

    Expression

    Bacterial

    Mutation

    fabH donor G954A

    Promoter

    T7/lac

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRD411

    Plasmid

    #178196

    Purpose

    sRok overexpression

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    srok
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRD231

    Plasmid

    #178195

    Purpose

    Rok overexpression

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    rok
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENT-L3-eBra-L4

    Plasmid

    #186368

    Purpose

    Entry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Brachury notochord enhancer
    

    Use

    Protein expression

    Promoter

    T7 promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENT-L3-Titf-L4

    Plasmid

    #186367

    Purpose

    Entry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Titf endoderm enhancer
    

    Use

    Protein expression

    Promoter

    T7 promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLS.543

    Plasmid

    #184960

    Purpose

    Test effect of a1/a2 length on editing murF using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
    

    Expression

    Bacterial

    Mutation

    murF donor G1359A

    Promoter

    T7/lac

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLS.545

    Plasmid

    #184962

    Purpose

    Test effect of a1/a2 length on editing priB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
    

    Expression

    Bacterial

    Mutation

    priB donor G1069A

    Promoter

    T7/lac

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-GFP-9S [rpoA]

    Plasmid

    #176221

    Purpose

    Expression of GFP-9S [rpoA]

    Depositor

    Ian Small

    Article

    Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

    Insert

    GFP-9S [rpoA]
    

    Use

    Synthetic Biology

    Tags

    His-GFP

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28b-pchD WT

    Plasmid

    #187973

    Purpose

    Expresses salicylate adenylation enzyme PchD from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Shelton et al J Biol Inorg Chem. 2022 May 5. pii: 10.1

    Insert

    pchD WT
    

    Tags

    Hexahistag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-eGFP-Pep-H

    Plasmid

    #185788

    Purpose

    Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    eGFP-Pep4
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-bhaA

    Plasmid

    #167810

    Purpose

    Expresses His6-tagged BhaA in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    BhaA
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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