We narrowed to 7,534 results for: ski

1. pDONR 223 VPS4A

   Plasmid

   #169319

   Purpose

   Gateway entry vector for human VPS4A ORF

   Depositor

   Francisca Vazquez

   Article

   Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

   Insert

   VPS4A (VPS4A Human)
   

   Use

   Gateway entry vector

   Promoter

   NA

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-46: MYL7-mEGFP

   Plasmid

   #114413

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

   Plasmid

   #112060

   Purpose

   Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

   Depositor

   Amy Palmer

   Article

   Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

   Insert

   NES-ZapCV2
   

   Tags

   Nuclear Export Signal (NES) derived from HIV-1

   Expression

   Mammalian

   Mutation

   ECFP gene is truncated 36 bp at 3' end. Venu…

   Promoter

   CMV

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-Dnmt3a3L-sc27

   Plasmid

   #71827

   Purpose

   For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

   Depositor

   Albert Jeltsch

   Article

   Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

   Insert

   Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-LIG1

   Plasmid

   #215131

   Purpose

   Expresses mCherry-LIG1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   LIG1 (LIG1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CIRTS-YTHDF2-control

   Plasmid

   #216857

   Purpose

   CIRTS RNA targeting system for targeted knockdown of m6A-containing transcript at the synapse. CIRTS-YTHDF2-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

   Insert

   CIRTS-YTHDF2-Calm3-U6-control gRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Syn1, U6

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe_puro_DEST_Flag_MDS1

   Plasmid

   #45258

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   MDS1 (MECOM, MDS1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 27, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pAAV.CAG-FLEX.iAChSnFR-Venus

   Plasmid

   #137959

   Purpose

   Expresses FLEXed iAChSnFR (yellow version) under CAG promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Insert

   iAChSnFR (yellow variant)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)

   Plasmid

   #55614

   Purpose

   Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
   

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   C4762 mutated to serine to render HERC2 catalytic…

   Available Since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_KEAP1_p.G333C

    Plasmid

    #82835

    Purpose

    Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    Gateway entry vector

    Mutation

    G333C; C624S

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_AKR1B1_WT_V5

    Plasmid

    #82953

    Purpose

    Gateway Donor vector containing AKR1B1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    AKR1B1 (AKR1B1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCjeCas9

    Plasmid

    #172215

    Purpose

    Expresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cells

    Depositor

    John van der Oost

    Article

    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

    Insert

    Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    

    Tags

    Histag

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-MPP8-10xHis

    Plasmid

    #194178

    Purpose

    Expresses MPP8-10xHis tagged

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Tags

    10xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_ARAF_WT

    Plasmid

    #82900

    Purpose

    Gateway Donor vector containing ARAF, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    NM_001654 (ARAF Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRCN0000338462

    Plasmid

    #78158

    Purpose

    shXPO1 targeting CDS

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    hU6 and hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223_TERT_p.L593F

    Plasmid

    #82856

    Purpose

    Gateway Donor vector containing TERT, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    TERT (TERT Human)
    

    Use

    Gateway entry vector

    Mutation

    D516G, L593F

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR 223 VPS4B

    Plasmid

    #169320

    Purpose

    Gateway entry vector for human VPS4B ORF

    Depositor

    Francisca Vazquez

    Article

    Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

    Insert

    VPS4B (VPS4B Human)
    

    Use

    Gateway entry vector

    Promoter

    NA

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-TRC.mKO2_shB3GNT5.1

    Plasmid

    #110325

    Purpose

    TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    B3GNT5 (B3GNT5 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CAG.iAChSnFR-NULL

    Plasmid

    #137956

    Purpose

    Expresses iAChSnFR (non-binding variant) under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR (non-binding variant)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Y140A binding site mutant

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p21-HA-NeoR

    Plasmid

    #215104

    Purpose

    Expresses p21-HA in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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