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V44 pHIPPY blunt

Plasmid

#15098

Depositor

Randall Moon

Article

Kaykas et al BMC Cell Biol. 2004 Apr 30;5:16. doi: 10

Type

Empty backbone

Use

RNAi

Expression

Mammalian

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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FRAP

Plasmid

#1827

Depositor

Philip Leder

Article

Ornitz et al Mol Cell Biol 1992 Jan;12(1):240-7.

Insert

FR1 AP (Fgfr1 Mouse)

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Tspan4

Plasmid

#210323

Purpose

Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

Depositor

Li Yu

Article

Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

Insert

Tetraspanin 4 (Tspan4 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_SFV_E1E2

Plasmid

#207308

Purpose

Mammalian expression of Semliki Forest virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SFV_E1E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_RV_G

Plasmid

#207303

Purpose

Mammalian expression of Rabies virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

RV_G (RABVgp4 Rabies virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-GA

Plasmid

#191666

Purpose

ddGFP-A-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Tags

HA and ddGFP-A

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDM_T7_HisLacZomega

Plasmid

#118815

Purpose

T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

lacZ_

Tags

histag

Expression

Bacterial

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp1

Plasmid

#72687

Purpose

Doxycyline regulated expression of Mesp1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 1 (MESP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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p18S.1 tag

Plasmid

#51729

Purpose

transcribes 18S containing hybridization tag

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pReadthroughUAG

Plasmid

#11511

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, UAG stop codon, Renilla luciferase, and 3' UTR short RNA binding sites

Use

Luciferase

Available Since

March 15, 2006

Availability

Academic Institutions and Nonprofits only
INS-2A-luciferase-2A-CD19-GFP

Plasmid

#159349

Purpose

Human INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Inserts

Luciferase-2A-human_CD19-2A-GFP
Luciferase-2A-human_CD19-2A-GFP (CD19 Human)

Use

Ins locus knockin donor vector

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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EID1-pGex-2TK

Plasmid

#22689

Depositor

William Kaelin

Article

Miyake et al Mol Cell Biol. 2000 Dec . 20(23):8889-90

Insert

E1A-like inhibitor of differentiation 1 (EID1 Human)

Tags

GST

Expression

Bacterial

Mutation

wt cDNA

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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HRE-oUnaG

Plasmid

#201712

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomaGCaMP7

Plasmid

#182942

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_CACNA2D2_OE_1

Plasmid

#161616

Purpose

Overexpresses CACNA2D2

Depositor

Mike McManus

Insert

CACNA2D2 (CACNA2D2 Human)

Tags

V5

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6-mTFP1-KanMX6

Plasmid

#69851

Purpose

C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ASAP2s

Plasmid

#101275

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

CAMKIIa

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-I3-01-Myc

Plasmid

#80038

Purpose

Expression of I3-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

I3-01-Myc

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Expression

Mammalian

Promoter

CAG

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pFM699

Plasmid

#74636

Purpose

alias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

mCherry

Expression

Yeast

Promoter

none

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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